Anti-EGFR Antibody

Cat: 10001-MM08

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Anti-EGFR Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-EGFR Antibody
Validated applications
Species reactivity
Reacts with: Human
Human EGFR
No cross-reactivity in ELISA with Human ErbB2 Human ErbB3 Human ErbB4
Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #08
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-EGFR Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:50-1:1000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-EGFR Antibody (Mouse Monoclonal antibody) Images
Human EGFR/HER1 Immunohistochemistry(IHC) 2938
Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human EGFR monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human EGFR/HER1 Flow Cytometry (FC) 5187
Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-EGFR Antibody Alternative Names
Anti-ERBB Antibody;Anti-ERBB1 Antibody;Anti-HER1 Antibody;Anti-mENA Antibody;Anti-NISBD2 Antibody;Anti-PIG61 Antibody
EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
epidermal growth factor receptor
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-T16
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 80100-T52
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 80100-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 51091-T52
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 51091-RP01
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R004
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA 51091-T48
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R112
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP02
    Anti-EGFR Antibody Mouse Monoclonal ELISA 10001-MM01
    Anti-EGFR Antibody (APC) Mouse Monoclonal FCM 10001-MM08-A
    Anti-EGFR Antibody (APC) Rabbit Monoclonal FCM 10001-RE01-A
    Anti-EGFR Antibody Rabbit Polyclonal ELISA,IHC-P 10001-T24
    Anti-EGFR Antibody Rabbit Polyclonal WB 100372-T32
    Anti-EGFR Antibody Rabbit Monoclonal Neutralization 10001-RE10
    Anti-EGFR Antibody (PE) Mouse Monoclonal FCM 10001-MM08-P
    Anti-EGFR Antibody (FITC) Mouse Monoclonal FCM 10001-MM08-F
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,FCM 10001-RE01
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,IHC-P,IHC-Fr 10001-R043
    Anti-EGFR Antibody Rabbit Monoclonal Neutralization 10001-REN01
    Anti-EGFR Antibody Mouse Monoclonal IHC-P 10001-MM11
    Anti-EGFR Antibody Rabbit Monoclonal ELISA(Cap) 10001-RE11
    Anti-EGFR Antibody (PE) Rabbit Monoclonal FCM 10001-RE01-P
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP01
    Anti-EGFR Antibody (FITC) Rabbit Monoclonal FCM 10001-RE01-F
    Anti-EGFR Antibody Rabbit Monoclonal WB,ELISA,ELISA(Det),ICC/IF,IF 10001-R021
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