|ELISA(Det)：||This antibody will detect Human EGFR / HER1 in ELISA pair set (Catalog: # SEK10001). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10001-RE11).|
EGFR was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate2 µL anti-EGFR rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-EGFR rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 134 kDa
Observed band size: 134 kDa
Anti-EGFR rabbit monoclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:180 kDa
Observed band size:180 kDa