Her2 / ERBB2 Protein, Cynomolgus, Recombinant (His Tag)

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Her2 / ERBB2 Protein, Cynomolgus, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA.
Immobilized Cynomolgus ERBB2-His at 10 μg/ml (100 μl/well) can bind Herceptin, The EC50 of Herceptin is 16.4-38.4 ng/ml.
Protein Construction
A DNA sequence encoding the cynomolgus ERBB2 (Met1-Thr652) [similar to Macaca fascicularis ERBB2 (EHH58073.1)] was expressed with a polyhistidinetag atthe C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Cynomolgus
Predicted N Terminal
Thr 23
Molecule Mass
The recombinant cynomolgus ERBB2 comprises 641 amino acids and has a calculated molecular mass of 70.8 KDa. The apparent molecular mass of it is approximately 80-105 KDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Her2 / ERBB2 Protein, Cynomolgus, Recombinant (His Tag): Images

Her2 / ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
  • Polyclonal Antibody against Different Extracellular Subdomains of HER2 Induces Tumor Growth Inhibition in vitro
    Author
    Hosseini Ghatar, R;Soltantoyeh, T;Bahadori, M;Khoshnoodi, J;Golsaz Shirazi, F;Jeddi Tehrani, M;Amiri, MM;Shokri, F;
    Year
    2017
    Journal
    Iran J Immunol
    Application
    Elisa
  • Epitope Mapping of Human HER2 Specific Mouse Monoclonal Antibodies Using Recombinant Extracellular Subdomains
    Author
    Hosseini Ghatar, R;Soltantoyeh, T;Bahadori, T;Golara, M;Hassannia, H;Khosravi Eghbal, R;Khoshnoodi, J;Judaki, MA;Golsaz-Shirazi, F;Jeddi-Tehrani, M;Amiri, MM;Shokri, F;
    Year
    2017
    Journal
    Asian Pac. J. Cancer Prev.
    Application
    binding
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