휴먼(Human) ESM1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

ESM1 cDNA Clone Datasheet

ESM1 cDNA Clone Product Information

• Gene Bank Ref.ID: M_007036.4
• cDNA Size: 555
• Gene Synonym: ESM1
• Source: Human

ESM1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens endothelial cell-specific molecule 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ESM1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ESM1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ESM1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGAGCG TCTTGCTGCT GACCACGCTC CTCGTGCCTG CACACCTGGT GGCCGCCTGG
61       AGCAATAATT ATGCGGTGGA CTGCCCTCAA CACTGTGACA GCAGTGAGTG CAAAAGCAGC
121      CCGCGCTGCA AGAGGACAGT GCTCGACGAC TGTGGCTGCT GCCGAGTGTG CGCTGCAGGG
181      CGGGGAGAAA CTTGCTACCG CACAGTCTCA GGCATGGATG GCATGAAGTG TGGCCCGGGG
241      CTGAGGTGTC AGCCTTCTAA TGGGGAGGAT CCTTTTGGTG AAGAGTTTGG TATCTGCAAA
301      GACTGTCCCT ACGGCACCTT CGGGATGGAT TGCAGAGAGA CCTGCAACTG CCAGTCAGGC
361      ATCTGTGACA GGGGGACGGG AAAATGCCTG AAATTCCCCT TCTTCCAATA TTCAGTAACC
421      AAGTCTTCCA ACAGATTTGT TTCTCTCACG GAGCATGACA TGGCATCTGG AGATGGCAAT
481      ATTGTGAGAG AAGAAGTTGT GAAAGAGAAT GCTGCCGGGT CTCCCGTAAT GAGGAAATGG
541      TTAAATCCAC GCTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).