Homo sapiens EFNA4 cDNA Clone

EFNA4 cDNA Clone Datasheet

EFNA4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_005227.2
• cDNA Size: 606
• Gene Synonym: EFNA4
• Source: Human

EFNA4 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens ephrin-A4 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the mutations 196 C/T resulting in the amino acid Pro substitution by Ser.

EFNA4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EFNA4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

EFNA4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG
61       CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT
121      CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC
181      TACGAAGGCC CAGGGTCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG
241      CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC
301      CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC
361      GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT
421      TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA GTCTGAGTCA
481      GCCCATCCTG TTGGGAGCCC TGGAGAGAGT GGCACATCAG GGTGGCGAGG GGGGGACACT
541      CCCAGCCCCC TCTGTCTCTT GCTATTACTG CTGCTTCTGA TTCTTCGTCT TCTGCGAATT
601      CTGTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).