Factor XIII cDNA ORF Clone in Cloning Vector, Human

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Factor XIII cDNA ORF Clone in Cloning Vector, Human: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
1986 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutation 493 A/G resulting in the amino acid Thr substitution by Ala.
Description
Full length Clone DNA of Human coagulation factor XIII, B polypeptide.
Plasmid
Vector
pMD18-T Vector
Sequencing Primers
M13-47 and RV-M
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Ampicillin
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

Factor XIII cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Factor XIII cDNA ORF Clone in Cloning Vector, Human: Alternative Names

Coagulation factor 13 cDNA ORF Clone, Human; Coagulation factor XIII cDNA ORF Clone, Human; FXIIIB cDNA ORF Clone, Human

Factor XIII Background Information

Coagulation factor XIII B chain, also known as Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain and F13B, is a secreted protein which contains 1 Sushi ( CCP / SCR ) domains. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Factor XIII acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Defects in F13B are the cause of factor XIII subunit B deficiency ( FA13BD ) which is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Full Name
coagulation factor XIII, B polypeptide
References
  • Bottenus R.E., et al.,(1990), Nucleotide sequence of the gene for the b subunit of human factor XIII. Biochemistry 29:11195-11209.
  • Ichinose A., et al., (1986), Amino acid sequence of the b subunit of human factor XIII, a protein composed of ten repetitive segments.Biochemistry 25:4633-4638.
  • Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.
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