휴먼(Human) FBXO15 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

FBXO15 cDNA Clone Datasheet

FBXO15 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_004333.4
• cDNA Size: 1305
• Gene Synonym: FBX15; MGC39671; FBXO15
• Source: Human

FBXO15 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens F-box protein 15 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 653T/C resulting in the amino acid Leu substitution by Pro.

FBXO15 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FBXO15 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

FBXO15 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCTTCAG AAATCTTGCT GAAGATATTT TCCTACTTGG ATGCTGTGAG CCTTCTGTGT
61       ACTGGATGTG TGAGCAGGCG CTTTTATCAT CTAGCCAATG ACAATTTTAT TTGGATCGGA
121      ATCTACTCAA CTGCTTTTTC ACCTGCAAGA TCAAATTGGA AATTTAATTC AGTAGAGAAG
181      ATAGCTATGT CTATGAGCTT TCTGTCAGTT CAGGATAAAG AAGCTGGTTA TTGGAAGAAA
241      GAATATATCA CAAAACAAAT AGCATCTGTA AAAGCCGCAC TAGCTGACAT TCTCAAACCT
301      GTCAACCCTT ACACAGGCCT TCCAGTTAAG ACCAAAGAGG CCCTCAGAAT ATTTGGTTTA
361      GGTTGGGCAA TTATACTGAA AGAAAAAGGT GGAAAAGAAT ATATCATGGA GCATGTTGAT
421      CTTTCCATAA ATGACACATC AGTTACTGTT ATATGGTATG GCAAAAAATG GCCATGCCTA
481      GCATCATTGT CAACCTTAGA TTTATGTGGC ATGACACCAG TTTTTACCGA CTGGTATAAA
541      ACTCCCACCA AACATAGACT CCGATGGCAT TCTTTAATTG CAAAGTACAA TCTGAGTCAT
601      TTGACCATAT CTACCATGAT TGGCTGTGAC AGACTCATTC GGATCTTCTG CCCGCACCCT
661      GGCCTCCTGG TGGGAGTGTG GAAGAAGGAG GAAGAACTGG CTTTTGTTAT GGCAAATCTT
721      CATTTTCATC ACCTTGTGGA GAGGAGCACA TTAGGCTCGG CTACTATCCC CTATGAACTG
781      CCTCCACATA GCCCCTTTTT GGATGATAGC CCCGAGTATG GACTGCACGG CTACCAACTC
841      CATGTTGATC TGCACAGCGG TGGGGTTTTC TACCTATGTG GTACATTTCG CAATCTCTTC
901      ACCAAGAGAG GAAATATTGA AAATGGACAT GTGAAGCTCA TTGTTATACA TTTAAAAAAT
961      AACAGAGAAC ACCTACCTCT TATTGGAAAA GTTGGCCTCT CGTGGAAAAC TGATATTTTT
1021     GATGGCTGTA TAAAGAGTTG TTCCATGATG GACGTAACTC TTTTGGATGA ACATGGGAAA
1081     CCCTTTTGGT GTTTCAGTTC CCCGGTGTGC CTGAGATCGC CTGCCACACC CTCTGACAGC
1141     TCTAGCTTCT TGGGACAGAC ATACAACGTG GACTACGTTG ATGCGGAAGG AAGAGTGCAC
1201     GTGGAGCTGG TGTGGATCAG AGAGACCGAA GAATACCTTA TTGTCAACCT GGTCCTTTAT
1261     CTTAGTATCG CAAAAATCAA CCATTGGTTT GGGACTGAAT ATTAG

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).