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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) FGF12 transcript variant 2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) FGF12 transcript variant 2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10312-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_004113.4
cDNA Size: 546
Synonym: FHF1, FGF12B
Source: Human. cDNA Description: ORF Clone of Homo sapiens fibroblast growth factor 12, transcript variant 2 DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 446 A/C causing the 149Gln to be replaced by Pro, and 24 A/C not causing any amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
Gene Bank Ref.ID: NM_004113.4
cDNA Size: 546
Synonym: FHF1, FGF12B
Source: Human. cDNA Description: ORF Clone of Homo sapiens fibroblast growth factor 12, transcript variant 2 DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 446 A/C causing the 149Gln to be replaced by Pro, and 24 A/C not causing any amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGGAGAGCA AAGAACCCCA GCTAAAAGGG ATTGTGACAA GGTTATTCAG CCAGCAGGGA
61 TACTTCCTGC AGATGCACCC AGATGGTACC ATTGATGGGA CCAAGGACGA AAACAGCGAC
121 TACACTCTCT TCAATCTAAT TCCCGTGGGC CTGCGTGTAG TGGCCATCCA AGGAGTGAAG
181 GCTAGCCTCT ATGTGGCCAT GAATGGTGAA GGCTATCTCT ACAGTTCAGA TGTTTTCACT
241 CCAGAATGCA AATTCAAGGA ATCTGTGTTT GAAAACTACT ATGTGATCTA TTCTTCCACA
301 CTGTACCGCC AGCAAGAATC AGGCCGAGCT TGGTTTCTGG GACTCAATAA AGAAGGTCAA
361 ATTATGAAGG GGAACAGAGT GAAGAAAACC AAGCCCTCAT CACATTTTGT ACCGAAACCT
421 ATTGAAGTGT GTATGTACAG AGAACAATCG CTACATGAAA TTGGAGAAAA ACAAGGGCGT
481 TCAAGGAAAA GTTCTGGAAC ACCAACCATG AATGGAGGCA AAGTTGTGAA TCAAGATTCA
541 ACATAG
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
