휴먼(Human) FLT3LG 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001459.2

cDNA Size:   708

Synonym:  FL

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens fms-related tyrosine kinase 3 ligand DNA.

Vector:  Vector pMD18-T Simple

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for nine point mutations: 1-9 ATGACAGTG/TGGACGATT.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        TGGACGATTC TGGCGCCAGC CTGGAGCCCA ACAACCTATC TCCTCCTGCT GCTGCTGCTG
61       AGCTCGGGAC TCAGTGGGAC CCAGGACTGC TCCTTCCAAC ACAGCCCCAT CTCCTCCGAC
121      TTCGCTGTCA AAATCCGTGA GCTGTCTGAC TACCTGCTTC AAGATTACCC AGTCACCGTG
181      GCCTCCAACC TGCAGGACGA GGAGCTCTGC GGGGGCCTCT GGCGGCTGGT CCTGGCACAG
241      CGCTGGATGG AGCGGCTCAA GACTGTCGCT GGGTCCAAGA TGCAAGGCTT GCTGGAGCGC
301      GTGAACACGG AGATACACTT TGTCACCAAA TGTGCCTTTC AGCCCCCCCC CAGCTGTCTT
361      CGCTTCGTCC AGACCAACAT CTCCCGCCTC CTGCAGGAGA CCTCCGAGCA GCTGGTGGCG
421      CTGAAGCCCT GGATCACTCG CCAGAACTTC TCCCGGTGCC TGGAGCTGCA GTGTCAGCCC
481      GACTCCTCAA CCCTGCCACC CCCATGGAGT CCCCGGCCCC TGGAGGCCAC AGCCCCGACA
541      GCCCCGCAGC CCCCTCTGCT CCTCCTACTG CTGCTGCCCG TGGGCCTCCT GCTGCTGGCC
601      GCTGCCTGGT GCCTGCACTG GCAGAGGACG CGGCGGAGGA CACCCCGCCC TGGGGAGCAG
661      GTGCCCCCCG TCCCCAGTCC CCAGGACCTG CTGCTTGTGG AGCACTGA

Quality Control:   cDNA sequencing results is available upon request.