휴먼(Human) sFRP-3/FRZB/FIZ 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001463.2

cDNA Size:   920

Synonym:  FRE, FZRB, Hfiz, FRITZ, FRP-3, FRZB1, SFRP3, SRFP3, FRZB-1, FRZB-PEN

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens frizzled-related protein DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: Please Inquire us)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for a 58bp (36-93) deletion.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGTCTGCG GCAGCCCGGG AGGGATGCTG CTGCTGCTGC AGCCTGTGAG CCCGTCCGCA
61       TCCCCCTGTG CAAGTCCCTG CCCTGGAACA TGACTAAGAT GCCCAACCAC CTGCACCACA
121      GCACTCAGGC CAACGCCATC CTGGCCATCG AGCAGTTCGA AGGTCTGCTG GGCACCCACT
181      GCAGCCCCGA TCTGCTCTTC TTCCTCTGTG CCATGTACGC GCCCATCTGC ACCATTGACT
241      TCCAGCACGA GCCCATCAAG CCCTGTAAGT CTGTGTGCGA GCGGGCCCGG CAGGGCTGTG
301      AGCCCATACT CATCAAGTAC CGCCACTCGT GGCCGGAGAA CCTGGCCTGC GAGGAGCTGC
361      CAGTGTACGA CAGGGGCGTG TGCATCTCTC CCGAGGCCAT CGTTACTGCG GACGGAGCTG
421      ATTTTCCTAT GGATTCTAGT AACGGAAACT GTAGAGGGGC AAGCAGTGAA CGCTGTAAAT
481      GTAAGCCTAT TAGAGCTACA CAGAAGACCT ATTTCCGGAA CAATTACAAC TATGTCATTC
541      GGGCTAAAGT TAAAGAGATA AAGACTAAGT GCCATGATGT GACTGCAGTA GTGGAGGTGA
601      AGGAGATTCT AAAGTCCTCT CTGGTAAACA TTCCACGGGA CACTGTCAAC CTCTATACCA
661      GCTCTGGCTG CCTCTGCCCT CCACTTAATG TTAATGAGGA ATATATCATC ATGGGCTATG
721      AAGATGAGGA ACGTTCCAGA TTACTCTTGG TGGAAGGCTC TATAGCTGAG AAGTGGAAGG
781      ATCGACTCGG TAAAAAAGTT AAGCGCTGGG ATATGAAGCT TCGTCATCTT GGACTCAGTA
841      AAAGTGATTC TAGCAATAGT GATTCCACTC AGAGTCAGAA GTCTGGCAGG AACTCGAACC
901      CCCGGCAAGC ACGCAACTAA

Quality Control:   cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).