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흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag 태그

데이터시트리뷰관련제품프로토콜
Ferret CA2 cDNA 클론 제품 정보
Gene_bank_ref_id:XM_004766964.1
cDNA 크기:783bp
cDNA 설명:Full length Clone DNA of Mustela putorius furo (sub-species: furo) carbonic anhydrase II with N terminal Flag tag.
유전자 동의어:CA2
:Ferret
벡터:pCMV3-N-FLAG
Plasmid:
제한 사이트:
태그 씨퀀스:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
염기서열 설명:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag 태그 on other vectors
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그FG60084-ACG265260
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그FG60084-ACR265260
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그FG60084-ANG265260
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그FG60084-ANR265260
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Flag 태그FG60084-CF229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-His 태그FG60084-CH229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Myc 태그FG60084-CM229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-HA 태그FG60084-CY229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone in cloning vectorFG60084-G88420
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag 태그FG60084-NF229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-His 태그FG60084-NH229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Myc 태그FG60084-NM229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-HA 태그FG60084-NY229890
흰족제비 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmidFG60084-UT229890
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연구배경

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

참고자료
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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    Cat No: FG60084-NF
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