휴먼(Human) GNS/G6S 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10319-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002076.3

cDNA Size:   1659

Synonym:  G6S, MGC21274

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens glucosamine (N-acetyl)-6-sulfatase DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the 198A/G mutation not resulting in amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCGGCTCC TGCCTCTAGC CCCAGGTCGG CTCCGGCGGG GCAGCCCCCG CCACCTGCCC
61       TCCTGCAGCC CAGCGCTGCT ACTGCTGGTG CTGGGCGGCT GCCTGGGGGT CTTCGGGGTG
121      GCTGCGGGAA CCCGGAGGCC CAACGTGGTG CTGCTCCTCA CGGACGACCA GGACGAAGTG
181      CTCGGCGGCA TGACACCGCT AAAGAAAACC AAAGCTCTCA TCGGAGAGAT GGGGATGACT
241      TTTTCCAGTG CTTATGTGCC AAGTGCTCTC TGCTGCCCCA GCAGAGCCAG TATCCTGACA
301      GGAAAGTACC CACATAATCA TCACGTTGTG AACAACACTC TGGAGGGGAA CTGCAGTAGT
361      AAGTCCTGGC AGAAGATCCA AGAACCAAAT ACTTTCCCAG CAATTCTCAG ATCAATGTGT
421      GGTTATCAGA CCTTTTTTGC AGGGAAATAT TTAAATGAGT ACGGAGCCCC AGATGCAGGT
481      GGACTAGAAC ACGTTCCTCT GGGTTGGAGT TACTGGTATG CCTTGGAAAA GAATTCTAAG
541      TATTATAATT ACACCCTGTC TATCAATGGG AAGGCACGGA AGCATGGTGA AAACTATAGT
601      GTGGACTACC TGACAGATGT TTTGGCTAAT GTCTCCTTGG ACTTTCTGGA CTACAAGTCC
661      AACTTTGAGC CCTTCTTCAT GATGATCGCC ACTCCAGCGC CTCATTCGCC TTGGACAGCT
721      GCACCTCAGT ACCAGAAGGC TTTCCAGAAT GTCTTTGCAC CAAGAAACAA GAACTTCAAC
781      ATCCATGGAA CGAACAAGCA CTGGTTAATT AGGCAAGCCA AGACTCCAAT GACTAATTCT
841      TCAATACAGT TTTTAGATAA TGCATTTAGG AAAAGGTGGC AAACTCTCCT CTCAGTTGAT
901      GACCTTGTGG AGAAACTGGT CAAGAGGCTG GAGTTCACTG GGGAGCTCAA CAACACTTAC
961      ATCTTCTATA CCTCAGACAA TGGCTATCAC ACAGGACAGT TTTCCTTGCC AATAGACAAG
1021     AGACAGCTGT ATGAGTTTGA TATCAAAGTT CCACTGTTGG TTCGAGGACC TGGGATCAAA
1081     CCAAATCAGA CAAGCAAGAT GCTGGTTGCC AACATTGACT TGGGTCCTAC TATTTTGGAC
1141     ATTGCTGGCT ACGACCTAAA TAAGACACAG ATGGATGGGA TGTCCTTATT GCCCATTTTG
1201     AGAGGTGCCA GTAACTTGAC CTGGCGATCA GATGTCCTGG TGGAATACCA AGGAGAAGGC
1261     CGTAACGTCA CTGACCCAAC ATGCCCTTCC CTGAGTCCTG GCGTATCTCA ATGCTTCCCA
1321     GACTGTGTAT GTGAAGATGC TTATAACAAT ACCTATGCCT GTGTGAGGAC AATGTCAGCA
1381     TTGTGGAATT TGCAGTATTG CGAGTTTGAT GACCAGGAGG TGTTTGTAGA AGTCTATAAT
1441     CTGACTGCAG ACCCAGACCA GATCACTAAC ATTGCTAAAA CCATAGACCC AGAGCTTTTA
1501     GGAAAGATGA ACTATCGGTT AATGATGTTA CAGTCCTGTT CTGGGCCAAC CTGTCGCACT
1561     CCAGGGGTTT TTGACCCCGG ATACAGGTTT GACCCCCGTC TCATGTTCAG CAATCGCGGC
1621     AGTGTCAGGA CTCGAAGATT TTCCAAACAT CTTCTGTAG

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) GNS/G6S 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)