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휴먼 GPR133/ADGRD1 Gene ORF cDNA clone in cloning vector

데이터시트리뷰관련제품프로토콜
Human GPR133 cDNA 클론 제품 정보
Gene_bank_ref_id:BC112309
cDNA 크기:2625bp
cDNA 설명:Full length Clone DNA of Homo sapiens G protein-coupled receptor 133.
유전자 동의어:PGR25
:Human
벡터:pGEM-T Vector
Plasmid:pGEM-GPR133
제한 사이트:
태그 씨퀀스:
염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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Cat No: HG15992-G
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