휴먼(Human) Galectin-3 transcript variant 1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

LGALS3 cDNA Clone Datasheet

LGALS3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002306.3
• cDNA Size: 753
• Gene Synonym: GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2Â
• Source: Human

LGALS3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens lectin, galactoside-binding, soluble, 3 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 292 A/C resulting in the amino acid Thr substitution by Pro.

LGALS3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LGALS3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LGALS3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGACA ATTTTTCGCT CCATGATGCG TTATCTGGGT CTGGAAACCC AAACCCTCAA
61       GGATGGCCTG GCGCATGGGG GAACCAGCCT GCTGGGGCAG GGGGCTACCC AGGGGCTTCC
121      TATCCTGGGG CCTACCCCGG GCAGGCACCC CCAGGGGCTT ATCCTGGACA GGCACCTCCA
181      GGCGCCTACC CTGGAGCACC TGGAGCTTAT CCCGGAGCAC CTGCACCTGG AGTCTACCCA
241      GGGCCACCCA GCGGCCCTGG GGCCTACCCA TCTTCTGGAC AGCCAAGTGC CCCCGGAGCC
301      TACCCTGCCA CTGGCCCCTA TGGCGCCCCT GCTGGGCCAC TGATTGTGCC TTATAACCTG
361      CCTTTGCCTG GGGGAGTGGT GCCTCGCATG CTGATAACAA TTCTGGGCAC GGTGAAGCCC
421      AATGCAAACA GAATTGCTTT AGATTTCCAA AGAGGGAATG ATGTTGCCTT CCACTTTAAC
481      CCACGCTTCA ATGAGAACAA CAGGAGAGTC ATTGTTTGCA ATACAAAGCT GGATAATAAC
541      TGGGGAAGGG AAGAAAGACA GTCGGTTTTC CCATTTGAAA GTGGGAAACC ATTCAAAATA
601      CAAGTACTGG TTGAACCTGA CCACTTCAAG GTTGCAGTGA ATGATGCTCA CTTGTTGCAG
661      TACAATCATC GGGTTAAAAA ACTCAATGAA ATCAGCAAAC TGGGAATTTC TGGTGACATA
721      GACCTCACCA GTGCTTCATA TACCATGATA TAA

 

Quality Control:cDNA sequencing results is available upon request.