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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) Granulysin transcript variant NKG5 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) Granulysin transcript variant NKG5 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10320-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_006433.3
cDNA Size: 438
Synonym: 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Source: Human. cDNA Description: ORF Clone of Homo sapiens granulysin, transcript variant NKG5 DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for a point mutation 356C/T causing the encoded amino acid 119Thr to be replaced by Ile. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
Gene Bank Ref.ID: NM_006433.3
cDNA Size: 438
Synonym: 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Source: Human. cDNA Description: ORF Clone of Homo sapiens granulysin, transcript variant NKG5 DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for a point mutation 356C/T causing the encoded amino acid 119Thr to be replaced by Ile. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGGCTACCT GGGCCCTCCT GCTCCTTGCA GCCATGCTCC TGGGCAACCC AGGTCTGGTC
61 TTCTCTCGTC TGAGCCCTGA GTACTACGAC CTGGCAAGAG CCCACCTGCG TGATGAGGAG
121 AAATCCTGCC CGTGCCTGGC CCAGGAGGGC CCCCAGGGTG ACCTGTTGAC CAAAACACAG
181 GAGCTGGGCC GTGACTACAG GACCTGTCTG ACGATAGTCC AAAAACTGAA GAAGATGGTG
241 GATAAGCCCA CCCAGAGAAG TGTTTCCAAT GCTGCGACCC GGGTGTGTAG GACGGGGAGG
301 TCACGATGGC GCGACGTCTG CAGAAATTTC ATGAGGAGGT ATCAGTCTAG AGTTATCCAG
361 GGCCTCGTGG CCGGAGAAAC TGCCCAGCAG ATCTGTGAGG ACCTCAGGTT GTGTATACCT
421 TCTACAGGTC CCCTCTGA
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
