마우스(Mouse) GREM2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

GREM2 cDNA Clone Datasheet

GREM2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011825.1
• cDNA Size: 507
• Gene Synonym: Prdc; Gremlin2
• Source: Mouse

GREM2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus gremlin 2 homolog, cysteine knot superfamily (Xenopus laevis) DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GREM2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GREM2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

GREM2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCTGGA AGCTCTCGCT GACCTTGCTC CTGGTGGCTG TGCTGGTAAA GGTAGCTGAA
61       ACACGGAAGA ACCGGCCTGC GGGCGCCATC CCCTCGCCTT ACAAGGATGG TAGCAGCAAC
121      AACTCAGAGA GGTGGCATCA CCAGATCAAG GAGGTGCTGG CCTCCAGCCA GGAGGCCCTG
181      GTAGTCACCG AGCGCAAGTA CCTCAAGAGT GACTGGTGCA AGACGCAGCC TCTGCGGCAG
241      ACAGTGAGCG AGGAGGGTTG CCGCAGCCGC ACCATCCTCA ACCGCTTCTG CTACGGCCAG
301      TGCAACTCCT TCTACATCCC GCGACACGTG AAGAAGGAGG AGGACTCCTT CCAATCCTGC
361      GCTTTCTGCA AGCCCCAGCG TGTCACCTCT GTCATCGTAG AGCTCGAATG CCCGGGTCTC
421      GACCCACCTT TCCGAATCAA GAAAATCCAG AAGGTGAAGC ATTGCCGGTG CATGTCAGTG
481      AACCTGAGTG ACTCCGACAA GCAGTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).