Influenza A H12N5 (A/green-winged teal/ALB/199/1991 ) HA cDNA 유전자 클론(gene clone) (codon optimized)

Influenza A H12N5 (A/green-winged teal/ALB/199/1991) cDNA Clone Datasheet

Influenza A H12N5 (A/green-winged teal/ALB/199/1991) cDNA Clone Product Information

• cDNA Size: 1717
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H12N5 (A/green-winged teal/ALB/199/1991) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H12N5 (A/green-winged teal/ALB/199/1991) HA DNA.
• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H12N5 (A/green-winged teal/ALB/199/1991) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H12N5 (A/green-winged teal/ALB/199/1991) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H12N5 (A/green-winged teal/ALB/199/1991) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGGA GAAGTTCATC ATCCTGAGCA CAGTGCTGGC TGTGTCCTTT
61       GCCTATGACA AGATTTGTAT TGGCTACCAG ACCAACAACA GCACAGAGAC AGTGAACACC
121      CTGATTGAAC AGAATGTGCC TGTGACCCAG GTGGAGGAAC TGGTTCATGG AGGCATTGAC
181      CCAATCCTGT GTGGCACAGA ACTGGGCAGC CCACTGGTGC TGGATGACTG TTCCTTGGAG
241      GGACTGATTC TGGGCAACCC AAAGTGTGAC CTCTACCTGA ATGGCAGGGA GTGGTCCTAC
301      ATTGTGGAGA GACCAAAGGA GATGGAGGGA GTGTGTTACC CTGGCAGCAT TGAGAACCAG
361      GAGGAACTGA GGTCCCTGTT CTCCAGCATC AAGAAATATG AGAGGGTGAA GATGTTTGAC
421      TTCACCAAGT GGAATGTGAC CTACACAGGC ACCAGCAAGG CTTGTAACAA CACCAGCAAC
481      CAGGGCTCCT TCTACAGGAG TATGAGGTGG CTGACCCTGA AATCTGGACA ATTTCCTGTC
541      CAGACAGATG AATACAAGAA CACCAGGGAC TCTGACATTG TGTTCACCTG GGCTATCCAC
601      CACCCTCCAA CCTCTGATGA ACAGGTGAAA CTCTACAAGA ACCCTGACAC CCTGTCCTCT
661      GTGACCACAG ATGAGATAAA CAGGTCCTTC AAGCCAAACA TTGGACCAAG ACCACTGGTG
721      AGGGGACAAC AGGGCAGGAT GGACTACTAC TGGGCTGTGC TGAAACCTGG ACAAACAGTG
781      AAGATTCAGA CCAATGGCAA CCTGATTGCC CCTGAATATG GACACCTGAT TACAGGCAAG
841      TCTCATGGCA GGATTCTGAA AAACAACCTG CCAATTGGAC AATGTGTGAC AGAGTGTCAA
901      CTTAATGAGG GAGTGATGAA CACCAGCAAG CCATTCCAGA ACACCAGCAA ACACTACATT
961      GGCAAGTGTC CAAAATACAT CCCATCTGGC TCCCTGAAAC TGGCTATTGG ACTGAGGAAT
1021     GTGCCACAGG TCCAGGACAG GGGACTGTTT GGAGCCATTG CTGGCTTCAT TGAGGGAGGC
1081     TGGCCTGGAC TGGTGGCTGG CTGGTATGGC TTCCAACACC AGAATGCTGA GGGCACAGGC
1141     ATTGCTGCTG ACAGGGACAG CACCCAGAAG GCTATTGACA ATATGCAGAA CAAACTGAAC
1201     AATGTGATTG ACAAGATGAA CAAGCAGTTT GAGGTGGTGA ACCATGAGTT CTCTGAGGTG
1261     GAGAGCAGGA TAAACACCAT CAACAGCAAG ATTGATGACC AGATTACAGA CATCTGGGCT
1321     TACAATGCTG AACTGCTGGT GCTGTTGGAG AACCAGAAGA CCCTGGATGA ACATGATGCC
1381     AATGTGAGGA ACCTCCATGA CAGGGTGAGG AGGGTGCTGA GGGAGAATGC CATTGACACA
1441     GGAGATGGCT GTTTTGAGAT TCTGCACAAG TGTGATGACA ACTGTATGGA CACCATCAGG
1501     AATGGCACCT ACAACCACAA GGAATATGAG GAGGAGAGCA AGATTGAGAG ACAGAAGATA
1561     AATGGAGTGA AATTGGAGGA GAACAGCACC TACAAGATTC TGAGCATCTA CTCCTCTGTG
1621     GCATCCTCCC TGGTGCTGCT GCTGATGATT ATTGGAGGCT TCATCTTTGG CTGTCAGAAT
1681     GGCAATGTGA GGTGTACCTT CTGTATTTAA ATCTAGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).