Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) HA cDNA 유전자 클론(gene clone) (codon optimized)

Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) cDNA Clone Datasheet

Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) cDNA Clone Product Information

• cDNA Size: 1720
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) HA DNA.
• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGGA CATCCCTGTG ATTGCCTTCC TGATTCTGAC CAGCACTTGT
61       GTCCAGGCTG ACAGGATTTG TGTGGGCTAC CTGAGCACCA ACTCCTCTGA GAGGGTGGAC
121      ACCCTGTTGG AGAACAATGT GCCTGTGACC TCCTCTGTGG ACCTGGTGGA GACCAACCAC
181      ACAGGCACCT ACTGTTCCCT GGGAGGCATC AGCCCTGTGC ATCTGGGAGA CTGTTCCTTT
241      GAGGGCTGGA TTGTGGGCAA CCCTGCCTGT GCCAGCAACC TGGGCATCAG GGAGTGGTCC
301      TACCTGATTG AGGACCCATC TGCCCCTCAT GGACTGTGTT ACCCTGGAGA ACTGGACAAC
361      AATGGAGAAC TGAGACACCT GTTCTCTGGC ATCAGGTCCT TCAGCAGGAC AGAACTGATT
421      GCCCCAACCT CCTGGGGAGC AGTGAATGAT GGAGTGTCCT CTGCCTGTCA GGACAAGGGA
481      GCCTCCTCCT TCTACAGGAA CCTGGTGTGG TTTGTGGAGA GGGGCAACAA ATACCCTGTG
541      ATTAGGGGCA CCTACAACAA CACCACAGGC AGGGATGTGC TGGTGATTTG GGGCATCCAC
601      CACCCTGTGA GCACAGATGA GGCAAGGAAA CTCTATGTGA ATGACAACCC ATACACCCTG
661      GTGAGCACCT CCTCCTGGAG CAGGAAATAC AACTTGGAGA TTGGCATCAG ACCTGGCTAC
721      AATGGACAAA AGTCCTGGAT GAAGATTTAC TGGTATCTGA TGCACCCTGG AGAGAGCATC
781      TCCTTTGAGA GCAATGGAGG ACTGCTGGCT CCAAAATATG GCTACATCAT TGAGGAATAT
841      GGCAAGGGCA GGATTTTCCA GAGCAGGATT AGGATTGCCA AGTGTAACAC CAAGTGTCAG
901      ACCTCTGTGG GAGGCATCAA CACCAACAAG ACCTTCCAGA ACATTGAGAG GAATGCCCTG
961      GGAGACTGTC CAAAATACAT CAAGTCTGGA CAACTTAAAC TGGCTACAGG ACTGAGGAAT
1021     GTGCCTGCCA TCAGCAAGAG GGGACTGTTT GGAGCCATTG CTGGCTTCAT TGAGGGAGGC
1081     TGGCCTGGAC TGATAAATGG CTGGTATGGC TTCCAACACC AGAATGAACA GGGAGTGGGG
1141     ATGGCTGCTG ACAAGGAGAG CACCCAGAAG GCTATTGACC AGATTACCAC CAAGATAAAC
1201     AACATCATTG AGAAGATGAA TGGCAACTAT GACAGCATCA GGGGAGAGTT CAGCCAGGTG
1261     GAACAGAGGA TAAATATGCT GGCTGACAGG ATTGATGATG CTGTGACAGA TGTGTGGTCC
1321     TACAATGCCA AACTGCTGGT GCTGTTGGAG AATGACAAGA CCCTGGATAT GCATGATGCC
1381     AATGTGAGGA ACCTCCATGA CCAGGTGAGG AGGGTGCTGA AAACCAATGC CATTGATGAG
1441     GGCAATGGCT GTTTTGAACT GCTGCACAAG TGTAATGACT CCTGTATGGA GACCATCAGG
1501     AATGGCACCT ACAACCACAC AGAATATGAG GAGGAGAGCA AACTGAAAAG ACAGGAGATT
1561     GAGGGCATCA AACTGAAATC TGATGACTCT GTCTACAAGG CTCTGAGCAT CTACTCCTGT
1621     ATTGCCTCCA GCATTGTGCT GGTGGGACTG ATTCTGGCTT TCATAATGTG GGCTTGTTCC
1681     TCTGGCAACT GTAGGTTCAA TGTGTGTATT TAAATCTAGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).