Influenza A H1N1 (A/Solomon Islands/3/2006) HA cDNA 유전자 클론(gene clone) (codon optimized)

Influenza A H1N1 (A/Solomon Islands/3/2006) cDNA Clone Datasheet

Influenza A H1N1 (A/Solomon Islands/3/2006) cDNA Clone Product Information

• cDNA Size: 1698
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H1N1 (A/Solomon Islands/3/2006) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H1N1 (A/Solomon Islands/3/2006) HA DNA.
• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H1N1 (A/Solomon Islands/3/2006) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H1N1 (A/Solomon Islands/3/2006) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H1N1 (A/Solomon Islands/3/2006) cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGTGA AACTGCTGGT GCTGCTGTGT ACCTTCACAG CCACCTATGC TGACACCATC
61       TGTATTGGCT ACCATGCCAA CAACAGCACA GACACAGTGG ACACAGTGTT GGAGAAGAAT
121      GTGACAGTGA CCCACTCTGT GAACCTGTTG GAGGACAGCC ACAATGGCAA ACTGTGTCTG
181      CTGAAAGGCA TTGCCCCACT CCAACTTGGC AACTGTTCTG TGGCTGGCTG GATTCTGGGC
241      AACCCTGAGT GTGAACTGCT GATTAGCAGG GAGTCCTGGT CCTACATTGT GGAGAAGCCA
301      AACCCTGAGA ATGGCACTTG TTACCCTGGA CACTTTGCTG ACTATGAGGA ACTGAGGGAA
361      CAACTTTCCT CTGTGTCCTC CTTTGAGAGG TTTGAGATTT TTCCAAAGGA GTCCTCCTGG
421      CCAAACCACA CAACGACAGG AGTGTCTGCC TCCTGTAGCC ACAATGGAGA GTCCTCCTTC
481      TACAAGAACC TGCTGTGGCT GACAGGCAAG AATGGACTCT ACCCAAACCT GAGCAAGTCC
541      TATGCCAACA ACAAGGAGAA GGAGGTGCTG GTGCTGTGGG GAGTGCATCA CCCTCCAAAC
601      ATTGGAGACC AGAGGGCTCT CTACCACAAA GAGAATGCCT ATGTGTCTGT GGTGTCCAGC
661      CACTACAGCA GGAAGTTCAC ACCTGAGATT GCCAAGAGAC CAAAGGTGAG GGACCAGGAG
721      GGCAGGATAA ACTACTACTG GACCCTGTTG GAACCTGGAG ACACCATCAT CTTTGAGGCT
781      AATGGCAACC TGATTGCCCC AAGATATGCC TTTGCCCTGA GCAGGGGCTT TGGCTCTGGC
841      ATCATCAACA GCAATGCCCC TATGGACGAG TGTGATGCCA AGTGTCAGAC ACCACAGGGA
901      GCCATCAACT CCTCCCTGCC ATTCCAGAAT GTGCATCCTG TGACCATTGG AGAGTGTCCA
961      AAATATGTGA GGTCTGCCAA ACTGAGGATG GTGACAGGAC TGAGGAACAT CCCAAGCATC
1021     CAGAGCAGGG GACTGTTTGG AGCCATTGCT GGCTTCATTG AGGGAGGCTG GACAGGGATG
1081     GTGGATGGCT GGTATGGCTA CCACCACCAG AATGAACAGG GCTCTGGCTA TGCTGCTGAC
1141     CAGAAGAGCA CCCAGAATGC CATCAATGGC ATCACCAACA AGGTGAACTC TGTGATTGAG
1201     AAGATGAACA CCCAGTTCAC AGCAGTGGGC AAGGAGTTCA ACAAATTGGA GAGGAGGATG
1261     GAGAACCTGA ACAAGAAGGT GGATGATGGC TTCATTGACA TCTGGACCTA CAATGCTGAA
1321     CTGCTGGTGC TGTTGGAGAA TGAGAGGACC CTGGACTTCC ATGACAGCAA TGTGAAGAAC
1381     CTCTATGAGA AGGTGAAGAG CCAACTTAAA AACAATGCCA AGGAGATTGG CAATGGCTGT
1441     TTTGAGTTCT ACCACAAGTG TAATGATGAG TGTATGGAGT CTGTGAAGAA TGGCACCTAT
1501     GACTACCCAA AATACTCTGA GGAGAGCAAA CTGAACAGGG AGAAGATTGA TGGAGTGAAA
1561     TTGGAGAGTA TGGGAGTCTA CCAGATTCTG GCTATCTACA GCACAGTGGC ATCCTCCCTG
1621     GTGCTGCTGG TGTCCCTGGG AGCCATCTCC TTCTGGATGT GTAGCAATGG CTCCCTCCAA
1681     TGTAGGATTT GTATTTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).