Influenza A H3N2 (A/Wisconsin/67/X-161/2005) HA cDNA 유전자 클론(gene clone) (codon optimized)

Influenza A H3N2 (A/Wisconsin/67/X-161/2005) cDNA Clone Datasheet

Influenza A H3N2 (A/Wisconsin/67/X-161/2005) cDNA Clone Product Information

• cDNA Size: 1701
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H3N2 (A/Wisconsin/67/X-161/2005) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H3N2 (A/Wisconsin/67/X-161/2005) HA DNA.
• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H3N2 (A/Wisconsin/67/X-161/2005) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H3N2 (A/Wisconsin/67/X-161/2005) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H3N2 (A/Wisconsin/67/X-161/2005) cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGACCA TCATTGCCCT GTCCTACATC CTGTGTCTGG TGTTCGCCCA GAAACTGCCT
61       GGCAATGACA ACAGCACAGC CACCCTGTGT CTGGGACACC ATGCTGTGCC AAATGGCACC
121      ATTGTGAAGA CCATCACCAA TGACCAGATT GAGGTGACCA ATGCCACAGA ACTGGTCCAG
181      TCCTCCAGCA CAGGAGGGAT TTGTGACAGC CCACACCAGA TTCTGGATGG AGAGAACTGT
241      ACCCTGATTG ATGCCCTGCT GGGAGACCCA CAGTGTGATG GCTTCCAGAA CAAGAAGTGG
301      GACCTGTTTG TGGAGAGGAG CAAGGCTTAC AGCAACTGTT ACCCATATGA TGTGCCTGAC
361      TATGCCTCCC TGAGGTCCCT GGTGGCATCC TCTGGCACCT TGGAGTTCAA CGATGAGTCC
421      TTCAACTGGA CAGGAGTGAC CCAGAATGGC ACCTCCTCTT CCTGTAAGAG GAGGAGCAAC
481      AACTCCTTCT TCAGCAGACT GAACTGGCTG ACCCAACTGA AGTTCAAATA CCCTGCCCTG
541      AATGTGACAA TGCCAAACAA TGAGAAGTTT GACAAACTCT ACATCTGGGG AGTGCATCAC
601      CCTGTCACAG ACAATGACCA GATTTTTCTA TATGCCCAGG CATCTGGCAG GATTACAGTG
661      AGCACCAAGA GGAGCCAACA GACAGTGATT CCAAACATTG GCAGCAGACC AAGGATCAGG
721      AACATCCCAA GCAGGATTAG CATCTACTGG ACCATTGTGA AGCCTGGAGA CATCCTGCTG
781      ATAAACAGCA CAGGCAACCT GATTGCCCCA AGGGGCTACT TCAAGATTAG GTCTGGCAAG
841      TCCAGCATAA TGAGGTCTGA TGCCCCAATT GGCAAGTGTA ACTCTGAGTG TATCACACCA
901      AATGGCAGCA TCCCAAATGA CAAGCCATTC CAGAATGTGA ACAGGATTAC CTATGGAGCC
961      TGTCCAAGAT ATGTGAAGCA GAACACCCTG AAACTGGCTA CAGGGATGAG GAATGTGCCT
1021     GAGAAGCAGA CCAGGGGCAT CTTTGGAGCC ATTGCTGGCT TCATTGAGAA TGGCTGGGAG
1081     GGGATGGTGG ATGGCTGGTA TGGCTTCAGA CACCAGAACT CTGAGGGCAT TGGACAAGCA
1141     GCAGACCTGA AAAGCACCCA GGCTGCCATT AACCAGATAA ATGGCAAACT GAACAGACTG
1201     ATTGGCAAGA CCAATGAGAA GTTCCACCAG ATTGAGAAGG AGTTCTCTGA GGTGGAGGGC
1261     AGGATTCAGG ACTTGGAGAA ATATGTGGAG GACACCAAGA TTGACCTGTG GTCCTACAAT
1321     GCTGAACTGC TGGTGGCTTT GGAGAACCAA CACACCATTG ACCTGACAGA CTCTGAGATG
1381     AACAAACTGT TTGAGAGGAC CAAGAAGCAA CTTAGGGAGA ATGCTGAGGA TATGGGCAAT
1441     GGCTGTTTCA AGATTTACCA CAAGTGTGAC AATGCCTGTA TTGGCAGCAT CAGGAATGGC
1501     ACCTATGACC ATGATGTCTA CAGGGATGAG GCTCTGAACA ACAGGTTCCA GATTAAGGGA
1561     GTGGAACTGA AATCTGGCTA CAAGGACTGG ATTCTGTGGA TTTCCTTTGC CATCTCCTGT
1621     TTCCTGCTGT GTGTGGCTCT GCTGGGCTTC ATAATGTGGG CTTGTCAGAA GGGCAACATC
1681     AGGTGTAACA TCTGTATTTA A

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).