H4N6 (A/Swine/Ontario/01911-1/99) 적혈구응집성(Hemagglutinin) (HA1 Subunit)

Influenza A H4N6 ( A/Swine/Ontario/01911-1/99 ) HA1 Protein

H4N6 ( A/Swine/Ontario/01911-1/99 ) HA1 Protein Product Information

• Synonym: HA1
• Protein Construction: A DNA sequence encoding the N-terminal segment ( Met 1 - Arg 343 ) of the influenza A hemagglutinin ( A/Swine/Ontario/01911-1/99 (H4N6) ) (AAG17429.1), termed as HA1 was expressed, fused with a C-terminal polyhistidine tag
• Source: Influenza A Virus H4N6
• Expression Host: Human Cells

H4N6 ( A/Swine/Ontario/01911-1/99 ) HA1 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 17
• Molecular Mass: The secreted recombinant influenza A H4N6 HA1 subunit (A/Swine/ Ontario/01911-1/99 (H4N6) ) comprises 338 amino acids and has a predicted molecular mass of 37.6 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 45-55 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: H4N6 HA1 protein

H4N6 ( A/Swine/Ontario/01911-1/99 ) HA1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Hemagglutinin Proteins
• H4N6 Hemagglutinin & Antibody
• A/Swine/Ontario/01911-1/99

H4N6 ( A/Swine/Ontario/01911-1/99 ) HA1 Protein Description

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). H4N6 is a subtype of Influenza A. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

1. Barman, S. et al., 2000, J. Virol. 74: 6538-45.
2. Suzuki, T. et al., 2005, J. Virol. 79: 11705-15.
3. Shinya K, et al., 2006, Nature. 440 (7083): 435-6.
4. Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-15.
5. von, Itzstein, M. 2007, Nat. Rev. Drug. Discov. 6: 967-74.
6. Christophe F, et al., 2009, Science. 324:1557-61