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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) HB-EGF 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) HB-EGF 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10325-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_001945.2
cDNA Size: 627
Synonym: DTR, DTS, DTSF, HEGFL
Source: Human. cDNA Description: ORF Clone of Homo sapiens heparin-binding EGF-like growth factor DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for a point mutation 273 C/T not causing amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
Gene Bank Ref.ID: NM_001945.2
cDNA Size: 627
Synonym: DTR, DTS, DTSF, HEGFL
Source: Human. cDNA Description: ORF Clone of Homo sapiens heparin-binding EGF-like growth factor DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for a point mutation 273 C/T not causing amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGAAGCTGC TGCCGTCGGT GGTGCTGAAG CTCTTTCTGG CTGCAGTTCT CTCGGCACTG
61 GTGACTGGCG AGAGCCTGGA GCGGCTTCGG AGAGGGCTAG CTGCTGGAAC CAGCAACCCG
121 GACCCTCCCA CTGTATCCAC GGACCAGCTG CTACCCCTAG GAGGCGGCCG GGACCGGAAA
181 GTCCGTGACT TGCAAGAGGC AGATCTGGAC CTTTTGAGAG TCACTTTATC CTCCAAGCCA
241 CAAGCACTGG CCACACCAAA CAAGGAGGAG CATGGGAAAA GAAAGAAGAA AGGCAAGGGG
301 CTAGGGAAGA AGAGGGACCC ATGTCTTCGG AAATACAAGG ACTTCTGCAT CCATGGAGAA
361 TGCAAATATG TGAAGGAGCT CCGGGCTCCC TCCTGCATCT GCCACCCGGG TTACCATGGA
421 GAGAGGTGTC ATGGGCTGAG CCTCCCAGTG GAAAATCGCT TATATACCTA TGACCACACA
481 ACCATCCTGG CCGTGGTGGC TGTGGTGCTG TCATCTGTCT GTCTGCTGGT CATCGTGGGG
541 CTTCTCATGT TTAGGTACCA TAGGAGAGGA GGTTATGATG TGGAAAATGA AGAGAAAGTG
601 AAGTTGGGCA TGACTAATTC CCACTGA
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
