휴먼(Human) HGFA 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10329-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001528.2

cDNA Size:   1968

Synonym:  HGFA, MGC138395, MGC138397

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens HGF activator DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the 693C/G point mutation resulting in the 231Phe substitution by Leu.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGGGCGCT GGGCCTGGGT CCCCAGCCCC TGGCCCCCAC CGGGGCTGGG CCCCTTCCTC
61       CTCCTCCTCC TGCTGCTGCT GCTGCTGCCA CGGGGGTTCC AGCCCCAGCC TGGCGGGAAC
121      CGTACGGAGT CCCCAGAACC TAATGCCACA GCGACCCCTG CGATCCCCAC TATCCTGGTG
181      ACCTCTGTGA CCTCTGAGAC CCCAGCAACA AGTGCTCCAG AGGCAGAGGG ACCCCAAAGT
241      GGGGGGCTCC CGCCCCCGCC CAGGGCAGTT CCCTCGAGCA GTAGCCCCCA GGCCCAAGCA
301      CTCACCGAGG ACGGGAGGCC CTGCAGGTTC CCCTTCCGCT ACGGGGGCCG CATGCTGCAT
361      GCCTGCACTT CGGAGGGCAG TGCACACAGG AAGTGGTGTG CCACAACTCA CAACTACGAC
421      CGGGACAGGG CCTGGGGCTA CTGTGTGGAG GCCACCCCGC CTCCAGGGGG CCCAGCTGCC
481      CTGGATCCCT GTGCCTCCGG CCCCTGCCTC AATGGAGGCT CCTGCTCCAA TACCCAGGAC
541      CCCCAGTCCT ATCACTGCAG CTGCCCCCGG GCCTTCACCG GCAAGGACTG CGGCACAGAG
601      AAATGCTTTG ATGAGACCCG CTACGAGTAC CTGGAGGGGG GCGACCGCTG GGCCCGCGTG
661      CGCCAGGGCC ACGTGGAACA GTGCGAGTGC TTGGGGGGCC GGACCTGGTG CGAAGGCACC
721      CGACATACAG CTTGTCTGAG CAGCCCTTGC CTGAACGGGG GCACCTGCCA CCTGATCGTG
781      GCCACCGGGA CCACCGTGTG TGCCTGCCCA CCAGGCTTCG CTGGACGGCT CTGCAACATC
841      GAGCCTGATG AGCGCTGCTT CTTGGGGAAC GGCACTGGGT ACCGTGGCGT GGCCAGCACC
901      TCAGCCTCGG GCCTCAGCTG CCTGGCCTGG AACTCCGATC TGCTCTACCA GGAGCTGCAC
961      GTGGACTCCG TGGGCGCCGC GGCCCTGCTG GGCCTGGGCC CCCATGCCTA CTGCCGGAAT
1021     CCGGACAATG ACGAGAGGCC CTGGTGCTAC GTGGTGAAGG ACAGCGCGCT CTCCTGGGAG
1081     TACTGCCGCC TGGAGGCCTG CGAATCCCTC ACCAGAGTCC AACTGTCACC GGATCTCCTG
1141     GCGACCCTGC CTGAGCCAGC CTCCCCGGGG CGCCAGGCCT GCGGCAGGAG GCACAAGAAG
1201     AGGACGTTCC TGCGGCCACG TATCATCGGC GGCTCCTCCT CGCTGCCCGG CTCGCACCCC
1261     TGGCTGGCCG CCATCTACAT CGGGGACAGC TTCTGCGCCG GGAGCCTGGT CCACACCTGC
1321     TGGGTGGTGT CGGCCGCCCA CTGCTTCTCC CACAGCCCCC CCAGGGACAG CGTCTCCGTG
1381     GTGCTGGGCC AGCACTTCTT CAACCGCACG ACGGACGTGA CGCAGACCTT CGGCATCGAG
1441     AAGTACATCC CGTACACCCT GTACTCGGTG TTCAACCCCA GCGACCACGA CCTCGTCCTG
1501     ATCCGGCTGA AGAAGAAAGG GGACCGCTGT GCCACACGCT CGCAGTTCGT GCAGCCCATC
1561     TGCCTGCCCG AGCCCGGCAG CACCTTCCCC GCAGGACACA AGTGCCAGAT TGCGGGCTGG
1621     GGCCACTTGG ATGAGAACGT GAGCGGCTAC TCCAGCTCCC TGCGGGAGGC CCTGGTCCCC
1681     CTGGTCGCCG ACCACAAGTG CAGCAGCCCT GAGGTCTACG GCGCCGACAT CAGCCCCAAC
1741     ATGCTCTGTG CCGGCTACTT CGACTGCAAG TCCGACGCCT GCCAGGGGGA CTCAGGGGGG
1801     CCCCTGGCCT GCGAGAAGAA CGGCGTGGCT TACCTCTACG GCATCATCAG CTGGGGTGAC
1861     GGCTGCGGGC GGCTCCACAA GCCGGGGGTC TACACCCGCG TGGCCAACTA TGTGGACTGG
1921     ATCAACGACC GGATACGGCC TCCCAGGCGG CTTGTGGCTC CCTCCTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) HGFA 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)