Anti-Histone H3 Antibody

Anti-Histone H3 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Histone H3 Antibody
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Histone H3
Immunogen
A synthetic peptide corresponding to the C-terminus of the human Histone H3.
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #1F6D7C4
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Histone H3 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1/1000-1/100000
IHC-P 1/50-1/1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Histone H3 Antibody (Mouse Monoclonal antibody) Images

Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
Immunochemical staining of human histone H3 in human colon carcinoma with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).

Anti-Histone H3 mouse monoclonal antibody at 1:2000 dilution

Lane A: Hela Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: 293 Whole Cell Lysate

Lysates/proteins at 30 μg per Lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/7500 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size: 18 kDa

Anti-Histone H3 mouse monoclonal antibody at 1:10000, 1:20000, 1:50000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Anti-Histone H3 mouse monoclonal antibody at 1:1000 dilution

Lane A: Histone H3 konckout Hela Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:18 kDa

Observed band size:18 kDa

Histone H3 Background Information

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.
References
  • Bhasin M, et al., 2006, 13(1): 102-12.
  • Lachner M, et al., 2001, 410(6824): 116-20.
  • Rosenfeld JA, et al., 2009, BMC Genomics. 10: 143.
  • Triazole-dithiocarbamate based selective lysine specific demethylase 1 (LSD1) inactivators inhibit gastric cancer cell growth, invasion, and migration
    Author
    Zheng, YC;Duan, YC;Ma, JL;Xu, RM;Zi, X;Lv, WL;Wang, MM;Ye, XW;Zhu, S;Mobley, D;Zhu, YY;Wang, JW;Li, JF;Wang, ZR;Zhao, W;Liu, HM;
    Year
    2013
    Journal
    J. Med. Chem.
  • Design, Synthesis, and Structure-Activity Relationship of Novel LSD1 Inhibitors Based on Pyrimidine-Thiourea Hybrids As Potent, Orally Active Antitumor Agents
    Author
    Ma, LY;Zheng, YC;Wang, SQ;Wang, B;Wang, ZR;Pang, LP;Zhang, M;Wang, JW;Ding, L;Li, J;Wang, C;Hu, B;Liu, Y;Zhang, XD;Wang, JJ;Wang, ZJ;Zhao, W;Liu, HM;
    Year
    2015
    Journal
    J. Med. Chem.
    Application
    WB
  • Point mutation impairs centromeric CENH3 loading and induces haploid plants
    Author
    Karimi-Ashtiyani, R;Ishii, T;Niessen, M;Stein, N;Heckmann, S;Gurushidze, M;Banaei-Moghaddam, AM;Fuchs, J;Schubert, V;Koch, K;Weiss, O;Demidov, D;Schmidt, K;Kumlehn, J;Houben, A;
    Year
    2015
    Journal
    Proc. Natl. Acad. Sci. U.S.A.
    Application
    WB
  • The Tet1 and histone methylation expression pattern in dairy goat testis
    Author
    Zheng, L;Zhu, H;Tang, F;Mu, H;Li, N;Wu, J;Hua, J;
    Year
    2015
    Journal
    Theriogenology
    Application
    IF and WB
  • The distribution of α-kleisin during meiosis in the holocentromeric plant Luzula elegans
    Author
    Ma, W;Schubert, V;Martis, MM;Hause, G;Liu, Z;Shen, Y;Conrad, U;Shi, W;Scholz, U;Taudien, S;Cheng, Z;Houben, A;
    Year
    2016
    Journal
    Chromosome Res.
    Application
    WB
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