HIV-1 (group M, subtype B, strain HXB2) nef ORF mammalian expression plasmid, N-His 태그

    데이터시트리뷰관련제품프로토콜
    HIV HIV-nef cDNA 클론 제품 정보
    Gene_bank_ref_id:K03455
    cDNA 크기:372bp
    cDNA 설명:Full length Clone DNA of HIV-1 (group M, subtype B, strain HXB2) nef with N terminal His tag.
    유전자 동의어:HIV-nef
    :HIV
    벡터:pCMV3-N-His
    Plasmid:
    제한 사이트:
    태그 씨퀀스:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at ambient temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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    Cat No: VG40249-NH
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