휴먼(Human) HMGB1/HMG1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002128.4

cDNA Size:   648

Synonym:  HMG1, HMG3, SBP-1, DKFZp686A04236

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens high-mobility group box 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for several point mutation, among which the 633T/C, 636 T/C do not cause any amino acid variation, and the stop codon TAA mutates to AAT encoding Asn.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CATCATATGC ATTTTTTGTG
61       CAAACTTGTC GGGAGGAGCA TAAGAAGAAG CACCCAGATG CTTCAGTCAA CTTCTCAGAG
121      TTTTCTAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAGAA AGGAAAATTT
181      GAAGATATGG CAAAAGCGGA CAAGGCCCGT TATGAAAGAG AAATGAAAAC CTATATCCCT
241      CCCAAAGGGG AGACAAAAAA GAAGTTCAAG GATCCCAATG CACCCAAGAG GCCTCCTTCG
301      GCCTTCTTCC TCTTCTGCTC TGAGTATCGC CCAAAAATCA AAGGAGAACA TCCTGGCCTG
361      TCCATTGGTG ATGTTGCGAA GAAACTGGGA GAGATGTGGA ATAACACTGC TGCAGATGAC
421      AAGCAGCCTT ATGAAAAGAA GGCTGCGAAG CTGAAGGAAA AATACGAAAA GGATATTGCT
481      GCATATCGAG CTAAAGGAAA GCCTGATGCA GCAAAAAAGG GAGTTGTCAA GGCTGAAAAA
541      AGCAAGAAAA AGAAGGAAGA GGAGGAAGAT GAGGAAGATG AAGAGGATGA GGAGGAGGAG
601      GAAGATGAAG AAGATGAAGA TGAAGAAGAA GACGACGATG ATGAAAAT

Quality Control:   cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).