휴먼(Human) HSPA2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

HSPA2 cDNA Clone Datasheet

HSPA2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_021979.3
• cDNA Size: 1920
• Gene Synonym: HSP70-2; HSP70-3; HSPA2
• Source: Human

HSPA2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens heat shock 70kDa protein 2 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for three mutations 684 C/T, 699 T/C and1278 G/A not causing the amino acid variation.

HSPA2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HSPA2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

HSPA2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTGCCC GTGGCCCGGC TATCGGCATC GACCTGGGCA CCACCTATTC GTGCGTCGGG
61       GTCTTCCAAC ATGGCAAGGT GGAGATCATC GCCAACGACC AGGGCAATCG CACCACCCCC
121      AGCTACGTGG CCTTCACGGA CACCGAGCGC CTCATCGGCG ACGCCGCCAA GAACCAGGTG
181      GCCATGAACC CCACCAACAC CATCTTCGAC GCCAAGAGGC TGATTGGACG GAAATTCGAG
241      GATGCCACAG TGCAGTCGGA TATGAAACAC TGGCCGTTCC GGGTGGTGAG CGAGGGAGGC
301      AAGCCCAAAG TGCAAGTAGA GTACAAGGGG GAGACCAAGA CCTTCTTCCC AGAGGAGATA
361      TCCTCCATGG TCCTCACGAA GATGAAGGAG ATCGCGGAAG CCTACCTGGG GGGCAAGGTG
421      CACAGCGCGG TCATAACGGT CCCGGCCTAT TTCAACGACT CGCAGCGCCA GGCCACCAAG
481      GACGCAGGCA CCATCACGGG GCTCAATGTG CTGCGCATCA TCAACGAGCC CACGGCGGCG
541      GCCATCGCCT ACGGCCTGGA CAAGAAGGGC TGCGCGGGCG GCGAGAAGAA CGTGCTCATC
601      TTTGACCTGG GCGGTGGCAC TTTCGACGTG TCCATCCTGA CCATCGAGGA TGGCATCTTC
661      GAGGTGAAGT CCACGGCCGG CGATACCCAC CTGGGCGGCG AGGACTTCGA CAACCGCATG
721      GTGAGCCACC TGGCGGAGGA GTTCAAGCGC AAGCACAAGA AGGACATTGG GCCCAACAAG
781      CGCGCCGTGA GGCGGCTGCG CACCGCTTGC GAGCGCGCCA AGCGCACCCT GAGCTCGTCC
841      ACGCAGGCGA GCATCGAGAT CGACTCGCTC TACGAGGGCG TGGACTTCTA TACGTCCATC
901      ACGCGCGCCC GCTTCGAGGA GCTCAATGCC GACCTCTTTC GCGGGACCCT GGAGCCGGTG
961      GAGAAGGCGC TGCGCGACGC CAAGCTGGAC AAGGGCCAGA TCCAGGAGAT CGTGCTGGTG
1021     GGCGGCTCCA CTCGTATCCC CAAGATCCAG AAGCTGCTGC AGGATTTCTT CAACGGCAAG
1081     GAGCTGAACA AGAGCATCAA CCCCGACGAG GCGGTGGCCT ATGGCGCCGC GGTGCAGGCG
1141     GCCATCCTCA TCGGCGACAA ATCAGAGAAT GTGCAGGACC TGCTGCTACT CGACGTGACC
1201     CCGTTGTCGC TGGGCATCGA GACAGCTGGC GGTGTCATGA CCCCACTCAT CAAGAGGAAC
1261     ACCACGATCC CCACCAAACA GACGCAGACC TTCACCACCT ACTCGGACAA CCAGAGCAGC
1321     GTACTGGTGC AGGTATACGA GGGCGAACGG GCCATGACCA AGGACAATAA CCTGCTGGGC
1381     AAGTTCGACC TGACCGGGAT TCCCCCTGCG CCTCGCGGGG TCCCCCAAAT CGAGGTTACC
1441     TTCGACATTG ACGCCAATGG CATCCTTAAC GTTACCGCCG CCGACAAGAG CACCGGTAAG
1501     GAAAACAAAA TCACCATCAC CAATGACAAA GGTCGTCTGA GCAAGGACGA CATTGACCGG
1561     ATGGTGCAGG AGGCGGAGCG GTACAAATCG GAAGATGAGG CGAATCGCGA CCGAGTCGCG
1621     GCCAAAAACG CCCTGGAGTC CTATACCTAC AACATCAAGC AGACGGTGGA AGACGAGAAA
1681     CTGAGGGGCA AGATTAGCGA GCAGGACAAA AACAAGATCC TCGACAAGTG TCAGGAGGTG
1741     ATCAACTGGC TCGACCGAAA CCAGATGGCA GAGAAAGATG AGTATGAACA CAAGCAGAAA
1801     GAGCTCGAAA GAGTTTGCAA CCCCATCATC AGCAAACTTT ACCAAGGTGG TCCTGGCGGC
1861     GGCAGCGGCG GCGGCGGTTC AGGAGCCTCC GGGGGACCCA CCATCGAAGA AGTGGACTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).