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휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-HA 태그

데이터시트리뷰관련제품프로토콜
Human CCL20 cDNA 클론 제품 정보
Gene_bank_ref_id:NM_001130046.1
cDNA 크기:288bp
cDNA 설명:Full length Clone DNA of Homo sapiens chemokine (C-C motif) ligand 20, transcript variant 2 with C terminal HA tag.
유전자 동의어:CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, CCL20
:Human
벡터:pCMV3-C-HA
Plasmid:
제한 사이트:
태그 씨퀀스:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
염기서열 설명:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-HA 태그 on other vectors
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG10485-ACG265260
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG10485-ACR265260
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Flag 태그HG10485-CF229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-His 태그HG10485-CH229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Myc 태그HG10485-CM229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, C-HA 태그HG10485-CY229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone in cloning vectorHG10485-M88420
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Flag 태그HG10485-NF229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, N-His 태그HG10485-NH229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Myc 태그HG10485-NM229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmid, N-HA 태그HG10485-NY229890
휴먼 CCL20/MIP-3 alpha/MIP3A transcript variant 2 Gene ORF cDNA clone expression plasmidHG10485-UT229890
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연구배경

Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC) or Macrophage Inflammatory Protein-3 (MIP3A) is a small cytokine belonging to the CC chemokine family that attracts immature dendritic cells and memory T lymphocytes, both expressing CCR6. Depending on the cell type, this chemokine was found to be inducible by cytokines (IL-1beta) and by bacterial, viral, or plant products (including LPS, dsRNA, and PMA). MIP3A / CCL20 is Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of MIP3A / CCL20 has been seen in thymus, prostate, testis, small intestine and colon. As a chemotactic factor, MIP3A / CCL20 attracts lymphocytes and, slightly, neutrophils, but not monocytes. This chemokine may Inhibit proliferation of myeloid progenitors in colony formation assays and it may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. Its C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis.

참고자료
  • Vicinus B, et al. (2012) miR-21 functionally interacts with the 3'UTR of chemokine CCL20 and down-regulates CCL20 expression in miR-21 transfected colorectal cancer cells. Cancer Lett. 316(1): 105-12.
  • Ding X, et al. (2012) High Expression of CCL20 Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma after Curative Resection. J Gastrointest Surg. 16(4): 828-36.
  • Ivison SM, et al. (2010) Oxidative stress enhances IL-8 and inhibits CCL20 production from intestinal epithelial cells in response to bacterial flagellin. Am J Physiol Gastrointest Liver Physiol. 299(3): 733-41.
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