Cofilin qPCR Primer Pairs, Human

Cat: HP103191
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Cofilin qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
Cofilin qPCR Primer Pairs, Human Alternative Names
CFL qPCR Primer Pairs, Human;HEL-S-15 qPCR Primer Pairs, Human
Cofilin Background Information

CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, α-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis.

Full Name
cofilin 1 (non-muscle)
References
  • Lappalainen P. et al., 1997, Nature. 388 (6637): 78-82.
  • Ichetovkin I. et al., 2000, Cell Motil. 45 (4): 293-306.
  • Carlier MF. et al., 1997, J Cell Biol. 136 (6): 1307-22.
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    Product Name Catalog#(PDF)
    Cofilin cDNA ORF Clone in Cloning Vector, Canine DG70184-U
    Cofilin cDNA ORF Clone, Canine, untagged DG70184-UT
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    Cofilin cDNA ORF Clone, Canine, C-GFPSpark® tag DG70184-ACG
    Cofilin cDNA ORF Clone, Canine, N-GFPSpark® tag DG70184-ANG
    Cofilin cDNA ORF Clone, Canine, C-OFPSpark® tag DG70184-ACR
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    Cofilin cDNA ORF Clone, Canine, N-DDK (Flag®) tag DG70184-NF
    Cofilin cDNA ORF Clone, Canine, C-His tag DG70184-CH
    Cofilin cDNA ORF Clone, Canine, N-His tag DG70184-NH
    Cofilin cDNA ORF Clone, Canine, C-HA tag DG70184-CY
    Cofilin cDNA ORF Clone, Canine, N-HA tag DG70184-NY
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    Cofilin qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
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    Cofilin cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90578-ACG
    Cofilin Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90578-ACGLN
    Cofilin cDNA ORF Clone, Rhesus, N-GFPSpark® tag CG90578-ANG
    Cofilin cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90578-ACR
    Cofilin cDNA ORF Clone, Rhesus, N-OFPSpark® tag CG90578-ANR
    Cofilin cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90578-CF
    Cofilin cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90578-NF
    Cofilin cDNA ORF Clone, Rhesus, C-His tag CG90578-CH
    Cofilin cDNA ORF Clone, Rhesus, N-His tag CG90578-NH
    Cofilin cDNA ORF Clone, Rhesus, C-HA tag CG90578-CY
    Cofilin cDNA ORF Clone, Rhesus, N-HA tag CG90578-NY
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    Cofilin qPCR Primer Pairs, Rat, Related Products

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    Cofilin qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    Cofilin cDNA ORF Clone in Cloning Vector, Mouse MG51445-G
    Cofilin cDNA ORF Clone, Mouse, untagged MG51445-UT
    Cofilin cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51445-ACG
    Cofilin Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51445-ACGLN
    Cofilin cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51445-ANG
    Cofilin cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51445-ACR
    Cofilin cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51445-ANR
    Cofilin cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51445-CF
    Cofilin cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51445-NF
    Cofilin cDNA ORF Clone, Mouse, C-His tag MG51445-CH
    Cofilin cDNA ORF Clone, Mouse, N-His tag MG51445-NH
    Cofilin cDNA ORF Clone, Mouse, C-HA tag MG51445-CY
    Cofilin cDNA ORF Clone, Mouse, N-HA tag MG51445-NY
    Cofilin cDNA ORF Clone, Mouse, C-Myc tag MG51445-CM
    Cofilin cDNA ORF Clone, Mouse, N-Myc tag MG51445-NM

    Cofilin qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    Cofilin cDNA ORF Clone in Cloning Vector, Human HG14544-G
    Cofilin cDNA ORF Clone, Human, untagged HG14544-UT
    Cofilin Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14544-ACGLN
    Cofilin cDNA ORF Clone, Human, C-GFPSpark® tag HG14544-ACG
    Cofilin cDNA ORF Clone, Human, N-GFPSpark® tag HG14544-ANG
    Cofilin cDNA ORF Clone, Human, C-OFPSpark® tag HG14544-ACR
    Cofilin cDNA ORF Clone, Human, N-OFPSpark® tag HG14544-ANR
    Cofilin cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14544-CF
    Cofilin cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14544-NF
    Cofilin cDNA ORF Clone, Human, C-His tag HG14544-CH
    Cofilin cDNA ORF Clone, Human, N-His tag HG14544-NH
    Cofilin cDNA ORF Clone, Human, C-HA tag HG14544-CY
    Cofilin cDNA ORF Clone, Human, N-HA tag HG14544-NY
    Cofilin cDNA ORF Clone, Human, C-Myc tag HG14544-CM
    Cofilin cDNA ORF Clone, Human, N-Myc tag HG14544-NM
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