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휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-Flag 태그

데이터시트리뷰관련제품프로토콜
Human CIRBP cDNA 클론 제품 정보
Gene_bank_ref_id:BC000403
cDNA 크기:519bp
cDNA 설명:Full length Clone DNA of Homo sapiens cold inducible RNA binding protein with N terminal Flag tag.
유전자 동의어:CIRP
:Human
벡터:pCMV3-N-FLAG
Plasmid:
제한 사이트:
태그 씨퀀스:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
염기서열 설명:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-Flag 태그 on other vectors
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG14578-ACG265260
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG14578-ACR265260
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG14578-ANG265260
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG14578-ANR265260
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, C-Flag 태그HG14578-CF229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, C-His 태그HG14578-CH229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, C-Myc 태그HG14578-CM229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, C-HA 태그HG14578-CY229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone in cloning vectorHG14578-G88420
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-Flag 태그HG14578-NF229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-His 태그HG14578-NH229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-Myc 태그HG14578-NM229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmid, N-HA 태그HG14578-NY229890
휴먼 CIRBP / Cold-inducible RNA-binding 단백질 Gene ORF cDNA clone expression plasmidHG14578-UT229890
 발현 벡터에 대해 자세히 알아보기
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연구배경

CIRBP, also known as cold-inducible RNA-binding protein, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP responds to a wide array of cellular stresses, including short wavelength ultraviolet light (UVC), at the transcriptional and post-translational level. It acts as a translational activator.CIRBP can bind the 3 translated region of specific transcripts to stabilize them and facilitate their transport to ribosomes for translation. CIRBP affects NF-κB signaling as opposed to IL1B mRNA stability directly.

참고자료
  • Artero-Castro A. et al., 2009, Mol Cell Biol. 29 (7): 1855-68.
  • Zeng Y. et al., 2009, J Cell Biochem. 107 (1): 179-88.
  • Brochu C. et al., 2013, PLoS One. 8 (2): e57426.
  • Size / Price
    Cat No: HG14578-NF
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