CRIPT cDNA ORF Clone, Human, N-DDK (Flag®) tag General Information
Full length Clone DNA of Human cysteine-rich PDZ-binding protein with N terminal Flag tag.
Enhanced CMV promoter
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
CRIPT cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
HSPC139 cDNA ORF Clone, Human;SSMDF cDNA ORF Clone, Human
CRIPT Background Information
CRIPT, also known as cysteine-rich PDZ-binding protein, belongs to the CRIPT family. It interacts with TUBB1. CRIPT also interacts strongly with the PDZ3 domain of members of the DLG4 family. It is involved in the cytoskeletal anchoring of DLG4 in excitatory synapses. CRIPT is highly conserved from mammals to plants and binds selectively to the third PDZ domain (PDZ3) of PSD-95 via its C terminus. n heterologous cells, CRIPT causes a redistribution of PSD-95 to microtubules. In brain, CRIPT colocalizes with PSD-95 in the postsynaptic density and can be coimmunoprecipitated with PSD-95 and tubulin. These findings suggest that CRIPT may regulate PSD-95 interaction with a tubulin-based cytoskeleton in excitatory synapses.
cysteine-rich PDZ-binding protein
Niethammer M. et al., 1998,Neuron. 20 (4): 693-707. Passafaro M. et al., 2000, Nat Neurosci. 2 (12): 1063-9. Piserchio A. et al., 2002, J Biol Chem. 277 (9): 6967-73. Fukunaga Y. et al., 2005, J Biochem. 138 (2): 177-82.