CTLA-4 cDNA ORF Clone, Human, N-HA tag General Information
Identical with the Gene Bank Ref. ID sequence.
Full length Clone DNA of Human cytotoxic T-lymphocyte-associated protein 4, transcript variant 1 with N terminal HA tag.
Enhanced CMV promoter
KpnI + XbaI(6kb+0.66kb)
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
CTLA-4 cDNA ORF Clone, Human, N-HA tag Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA Tag monoclonal antibody (100028-MM10) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
CTLA-4 cDNA ORF Clone, Human, N-HA tag Alternative Names
ALPS5 cDNA ORF Clone, Human;CD cDNA ORF Clone, Human;CD152 cDNA ORF Clone, Human;CELIAC3 cDNA ORF Clone, Human;CTLA-4 cDNA ORF Clone, Human;GRD4 cDNA ORF Clone, Human;GSE cDNA ORF Clone, Human;IDDM12 cDNA ORF Clone, Human
CTLA-4 Background Information
cytotoxic T-lymphocyte-associated protein 4
Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.