휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-Flag 태그

    데이터시트리뷰관련제품프로토콜
    휴먼 CYP2J2 cDNA 클론 제품 정보
    Gene_bank_ref_id:BC032594
    cDNA 크기:1509bp
    cDNA 설명:Full length Clone DNA of Homo sapiens cytochrome P450, family 2, subfamily J, polypeptide 2 with C terminal Flag tag.
    유전자 동의어:CPJ2
    :Human
    벡터:pCMV3-C-FLAG
    Plasmid:
    제한 사이트:
    태그 씨퀀스:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    염기서열 설명:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CYP2J2 qPCR primers for gene expression analysis, HP103710 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-Flag 태그 on other vectors
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG15082-ACG288840
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG15082-ACR288840
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG15082-ANG288840
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG15082-ANR288840
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-Flag 태그HG15082-CF253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-His 태그HG15082-CH253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-Myc 태그HG15082-CM253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, C-HA 태그HG15082-CY253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone in cloning vectorHG15082-G88420
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, N-Flag 태그HG15082-NF253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, N-His 태그HG15082-NH253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, N-Myc 태그HG15082-NM253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmid, N-HA 태그HG15082-NY253470
    휴먼 CYP2J2/Cytochrome p450 2J2 Gene ORF cDNA clone expression plasmidHG15082-UT253470
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    Cat No: HG15082-CF
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