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휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-Myc 태그

데이터시트리뷰관련제품프로토콜
Human ECD cDNA 클론 제품 정보
Gene_bank_ref_id:BC000721
cDNA 크기:1935bp
cDNA 설명:Full length Clone DNA of Homo sapiens ecdysoneless homolog (DrosophilA) with N terminal Myc tag.
유전자 동의어:GCR2, HSGT1, ECD
:Human
벡터:pCMV3-N-Myc
Plasmid:
제한 사이트:
태그 씨퀀스:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
염기서열 설명:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-Myc 태그 on other vectors
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG14311-ACG288840
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG14311-ACR288840
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG14311-ANG288840
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG14311-ANR288840
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, C-Flag 태그HG14311-CF253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, C-His 태그HG14311-CH253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, C-Myc 태그HG14311-CM253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, C-HA 태그HG14311-CY253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone in cloning vectorHG14311-G88420
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-Flag 태그HG14311-NF253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-His 태그HG14311-NH253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-Myc 태그HG14311-NM253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmid, N-HA 태그HG14311-NY253470
휴먼 ECD / Ecdysoneless homolog Gene ORF cDNA clone expression plasmidHG14311-UT253470
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연구배경

ECD, also known as ecdysoneless homolog, belongs to the SGT1 family. It is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. This function is related to its ability to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors. It is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. ECD may also be a transcriptional activator required for the expression of glycolytic genes.

참고자료
  • Badzek S. et al., 2011, Wien Klin Wochenschr. 123 (23-24): 726-31.
  • Zhao X. et al., 2012, Breast Cancer Res Treat. 134 (1): 171-80.
  • Dey P. et al., 2012, Clin Cancer Res. 18 (22): 6188-98.
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    Cat No: HG14311-NM
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