휴먼 EDNRB / Endothelin B Receptor Gene ORF cDNA clone expression plasmid, N-Myc 태그

  • Ferret CD3e/CD3 epsilon Gene Plasmid Map 5630
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휴먼 EDNRB cDNA 클론 제품 정보
Gene_bank_ref_id:BC014472
cDNA 크기:1344 bp
cDNA 설명:Full length Clone DNA of Homo sapiens endothelin receptor type B with N terminal Myc tag.
유전자 동의어:ETB, ET-B, ETBR, ETRB, HSCR, WS4A, ABCDS, ET-BR, HSCR2
:Human
벡터:pCMV3-SP-N-Myc
Plasmid:pCMV3-SP-Myc-EDNRB
제한 사이트:KpnI + XbaI(6kb+1.34kb)
태그 씨퀀스:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with EDNRB qPCR primers for gene expression analysis, HP104096 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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Cat No: HG15473-NM
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