GCSH cDNA ORF Clone, Human, N-DDK (Flag®) tag General Information
Full length Clone DNA of Human glycine cleavage system protein H (aminomethyl carrier) with N terminal Flag tag.
Enhanced CMV promoter
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
GCSH cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
GCE cDNA ORF Clone, Human;NKH cDNA ORF Clone, Human
GCSH Background Information
Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in GCSH gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for GCSH gene. Also, several transcribed and non-transcribed pseudogenes of GCSH gene exist throughout the genome.
glycine cleavage system protein H (aminomethyl carrier)
Hiraga K. et al., 1988, Biochem Biophys Res Commun. 151 (2): 758-62. Fujiwara K. et al., 1991, Biochem Biophys Res Commun. 176 (2): 711-6. Koyata H. et al., 1991, Am J Hum Genet. 48 (2): 351-61.