휴먼 MDA5/IFIH1 Gene ORF cDNA clone expression plasmid, N-His 태그

  • Ferret TNF-alpha/TNFA/TNFSF2 Gene Plasmid Map 5629
데이터시트리뷰관련제품프로토콜
휴먼 IFIH1 cDNA 클론 제품 정보
Gene_bank_ref_id:BC046208
cDNA 크기:711 bp
cDNA 설명:Full length Clone DNA of Homo sapiens interferon induced with helicase C domain 1 with N terminal His tag.
유전자 동의어:Hlcd, MDA5, MDA-5, RLR-2, IDDM19
:Human
벡터:pCMV3-N-His
Plasmid:pCMV3-His-IFIH1
제한 사이트:KpnI + XbaI(6kb+0.71kb)
태그 씨퀀스:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with IFIH1 qPCR primers for gene expression analysis, HP104013 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Cat No: HG15389-NH
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