IL-6 ELISA Kit, Human

1/2
Price:
Size:
Number:

IL-6 ELISA Kit, Human: General Information

Product name
IL-6 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
0.69 pg/mL
Assay range
3.13-200 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-6
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL6 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 18.00 37.00 79.00
SD 0.66 1.12 1.92
CV(%) 3.6% 3.0% 2.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 20.00 39.00 82.00
SD 2.00 2.65 4.62
CV(%) 10.0% 6.8% 5.7%
Recovery
The recovery of Human IL6 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
75
Range
72-78%
Sample
supernatant (n=3)
Average % Recovery
110
Range
97-117%
Linearity
Supernatant
  Recovery of detected
1:2 96%
1:4 104%
1:8 98%
1:16 98%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-6 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-6 protein in Serum,Cell supernatant . It contains recombinant Human IL-6, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-6 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL6. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-6 ELISA Kit, Human: Alternative Names

BSF2 ELISA Kit, Human; HGF ELISA Kit, Human; HSF ELISA Kit, Human; IFNB2 ELISA Kit, Human; IL-6 ELISA Kit, Human; Interleukin-6 ELISA Kit, Human

IL-6 Background Information

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 13 (gp13) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Full Name
interleukin 6
References
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
  • LncRNA-MEG3 alleviates high glucose induced inflammation and apoptosis of retina epithelial cells via regulating miR-34a/SIRT1 axis
    Author
    Tong, P;Peng, QH;Gu, LM;Xie, WW;Li, WJ;
    Year
    2018
    Journal
    Exp. Mol. Pathol.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait