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휴먼 MLH1 Gene ORF cDNA clone expression plasmid, C-His 태그

데이터시트리뷰관련제품프로토콜
Human MLH1 cDNA 클론 제품 정보
Gene_bank_ref_id:NM_000249
cDNA 크기:2271bp
cDNA 설명:Full length Clone DNA of Homo sapiens mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) with C terminal His tag.
유전자 동의어:HNPCC, FCC2, HNPCC2
:Human
벡터:pCMV3-C-His
Plasmid:pCMV3-MLH1-His
제한 사이트:KpnI+XbaI (6kb+2.32kb)
태그 씨퀀스:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
Human MLH1 Gene Plasmid Map
Human MLH1 ORF mammalian expression plasmid, C-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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Cat No: HG12041-CH
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  • Human MLH1 ORF mammalian expression plasmid, C-His tag
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