MMGT1 cDNA ORF Clone, Human, C-His tag General Information
Full length Clone DNA of Human membrane magnesium transporter 1 with C terminal His tag.
Enhanced CMV promoter
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
MMGT1 cDNA ORF Clone, Human, C-His tag Alternative Names
EMC5 cDNA ORF Clone, Human;TMEM32 cDNA ORF Clone, Human
MMGT1 Background Information
MMGT1, also known as EMC5, is comparable with primary microglial cells with respect to morphology, presence of acetylated low density lipoprotein receptor, non-specific esterase, CD63, major histocompatibility complex antigens and CD11, and binding for Ricinus communis agglutinin. Primary microglia as well as MMGT1 cells are negative for glial fibrillary acidic protein. Different MMGT1 strains are obtained after subcloning, two of which resembled histiocytes (F4/80 and BM-8). These cell strains, MMGT12 and 16, are able to opsonize latex beads, and could be induced by endotoxins (LPS) to secrete TNF-alpha, IL-1, IL-6, TGF-beta, and EGF.
membrane magnesium transporter 1
Goytain A. et al., 2008, Am J Physiol Cell Physiol. 294 (2): C495-502. Briers TW. et al., 1994, J Neuroimmunol. 52 (2): 153-64. Jäger S. et al., 2011, Nature. 481 (7381): 365-70.