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휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Flag 태그

데이터시트리뷰관련제품프로토콜
Human PMVK cDNA 클론 제품 정보
Gene_bank_ref_id:BC006089
cDNA 크기:579bp
cDNA 설명:Full length Clone DNA of Homo sapiens phosphomevalonate kinase with N terminal Flag tag.
유전자 동의어:PMK, PMKA, PMKASE, HUMPMKI
:Human
벡터:pCMV3-N-FLAG
Plasmid:
제한 사이트:
태그 씨퀀스:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
염기서열 설명:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Flag 태그 on other vectors
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG14583-ACG265260
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG14583-ACR265260
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG14583-ANG265260
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG14583-ANR265260
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-Flag 태그HG14583-CF229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-His 태그HG14583-CH229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-Myc 태그HG14583-CM229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-HA 태그HG14583-CY229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone in cloning vectorHG14583-G88420
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Flag 태그HG14583-NF229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-His 태그HG14583-NH229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Myc 태그HG14583-NM229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-HA 태그HG14583-NY229890
휴먼 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmidHG14583-UT229890
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연구배경

PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.

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Cat No: HG14583-NF
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