|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그||HG11258-ACG|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그||HG11258-ACR|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, C-Flag 태그||HG11258-CF|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, C-His 태그||HG11258-CH|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, C-Myc 태그||HG11258-CM|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, C-HA 태그||HG11258-CY|
|휴먼 TPST1 Gene ORF cDNA clone in cloning vector||HG11258-M|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, N-Flag 태그||HG11258-NF|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, N-His 태그||HG11258-NH|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, N-Myc 태그||HG11258-NM|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid, N-HA 태그||HG11258-NY|
|휴먼 TPST1 Gene ORF cDNA clone expression plasmid||HG11258-UT|
|발현 벡터에 대해 자세히 알아보기|
Protein-tyrosine sulfotransferase 1, also known as Tyrosylprotein sulfotransferase 1 and TPST1, is a single-pass type I I membrane protein which belongs to the protein sulfotransferase family. Tyrosine O-sulfation is a common posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called tyrosylprotein sulfotransferase (TPST) that catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to tyrosine residues within acidic motifs of polypeptides. Tyrosine O-sulfation has been shown to be important in protein-protein interactions in several systems. Tyrosine sulfation is mediated by one of two Golgi isoenzymes, called tyrosylprotein sulfotransferases (TPST-1 and TPST-2). A relatively small number of proteins are known to undergo tyrosine sulfation, including certain adhesion molecules, G-protein-coupled receptors, coagulation factors, serpins, extracellular matrix proteins, and hormones. TPST1 is a human tyrosylprotein sulfotransferase that uses 3'phosphoadenosine-5'phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. TPST1 bears N-linked glycosyl residues exclusively at position Asn60 and Asn262. TPST1 and TPST2 have distinct biological roles that may reflect differences in their macromolecular substrate specificity.