휴먼(Human) IFNA7 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_021057.2

cDNA Size:   570

Synonym:  IFNA-J

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens interferon, alpha 7 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGCCCGGT CCTTTTCTTT ACTGATGGTC GTGCTGGTAC TCAGCTACAA ATCCATCTGC
61       TCTCTGGGCT GTGATCTGCC TCAGACCCAC AGCCTGCGTA ATAGGAGGGC CTTGATACTC
121      CTGGCACAAA TGGGAAGAAT CTCTCCTTTC TCCTGCTTGA AGGACAGACA TGAATTCAGA
181      TTCCCAGAGG AGGAGTTTGA TGGCCACCAG TTCCAGAAGA CTCAAGCCAT CTCTGTCCTC
241      CATGAGATGA TCCAGCAGAC CTTCAATCTC TTCAGCACAG AGGACTCATC TGCTGCTTGG
301      GAACAGAGCC TCCTAGAAAA ATTTTCCACT GAACTTTACC AGCAACTGAA TGACCTGGAA
361      GCATGTGTGA TACAGGAGGT TGGGGTGGAA GAGACTCCCC TGATGAATGA GGACTTCATC
421      CTGGCTGTGA GGAAATACTT CCAAAGAATC ACTCTTTATC TAATGGAGAA GAAATACAGC
481      CCTTGTGCCT GGGAGGTTGT CAGAGCAGAA ATCATGAGAT CCTTCTCTTT TTCAACAAAC
541      TTGAAAAAAG GATTAAGGAG GAAGGATTGA

Quality Control:   cDNA sequencing results is available upon request.