휴먼(Human) IFNÏ‰ 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10353-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002177.1

cDNA Size:   588

Synonym:  IFNW1

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens interferon, omega 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGCCCTCC TGTTCCCTCT ACTGGCAGCC CTAGTGATGA CCAGCTATAG CCCTGTTGGA
61       TCTCTGGGCT GTGATCTGCC TCAGAACCAT GGCCTACTTA GCAGGAACAC CTTGGTGCTT
121      CTGCACCAAA TGAGGAGAAT CTCCCCTTTC TTGTGTCTCA AGGACAGAAG AGACTTCAGG
181      TTCCCCCAGG AGATGGTAAA AGGGAGCCAG TTGCAGAAGG CCCATGTCAT GTCTGTCCTC
241      CATGAGATGC TGCAGCAGAT CTTCAGCCTC TTCCACACAG AGCGCTCCTC TGCTGCCTGG
301      AACATGACCC TCCTAGACCA ACTCCACACT GGACTTCATC AGCAACTGCA ACACCTGGAG
361      ACCTGCTTGC TGCAGGTAGT GGGAGAAGGA GAATCTGCTG GGGCAATTAG CAGCCCTGCA
421      CTGACCTTGA GGAGGTACTT CCAGGGAATC CGTGTCTACC TGAAAGAGAA GAAATACAGC
481      GACTGTGCCT GGGAAGTTGT CAGAATGGAA ATCATGAAAT CCTTGTTCTT ATCAACAAAC
541      ATGCAAGAAA GACTGAGAAG TAAAGATAGA GACCTGGGCT CATCTTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) IFNÏ‰ 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)