휴먼(Human) IFNA10 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002171.1

cDNA Size:   570

Synonym:  MGC119878, MGC119879

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens interferon, alpha 10 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for two point mutations: 66G/A and 96C/G, neither of which results in amino acid variation.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGCCCTGT CCTTTTCTTT ACTTATGGCC GTGCTGGTGC TCAGCTACAA ATCCATCTGT
61       TCTCTAGGCT GTGATCTGCC TCAGACCCAC AGCCTGGGTA ATAGGAGGGC CTTGATACTC
121      CTGGGACAAA TGGGAAGAAT CTCTCCTTTC TCCTGCCTGA AGGACAGACA TGATTTCCGA
181      ATCCCCCAGG AGGAGTTTGA TGGCAACCAG TTCCAGAAGG CTCAAGCCAT CTCTGTCCTC
241      CATGAGATGA TCCAGCAGAC CTTCAATCTC TTCAGCACAG AGGACTCATC TGCTGCTTGG
301      GAACAGAGCC TCCTAGAAAA ATTTTCCACT GAACTTTACC AGCAACTGAA TGACCTGGAA
361      GCATGTGTGA TACAGGAGGT TGGGGTGGAA GAGACTCCCC TGATGAATGA GGACTCCATC
421      CTGGCTGTGA GGAAATACTT CCAAAGAATC ACTCTTTATC TAATAGAGAG GAAATACAGC
481      CCTTGTGCCT GGGAGGTTGT CAGAGCAGAA ATCATGAGAT CCCTCTCGTT TTCAACAAAC
541      TTGCAAAAAA GATTAAGGAG GAAGGATTGA

Quality Control:   cDNA sequencing results is available upon request.