휴먼(Human) IFNαB2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002170.3

cDNA Size:   570

Synonym:  IFNA8

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens interferon, alpha 8 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the 27C/G mutation not resulting in amino acid variation.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGCCTTGA CTTTTTATTT ACTGGTGGCC CTAGTGGTGC TCAGCTACAA GTCATTCAGC
61       TCTCTGGGCT GTGATCTGCC TCAGACTCAC AGCCTGGGTA ACAGGAGGGC CTTGATACTC
121      CTGGCACAAA TGCGAAGAAT CTCTCCTTTC TCCTGCCTGA AGGACAGACA TGACTTTGAA
181      TTCCCCCAGG AGGAGTTTGA TGATAAACAG TTCCAGAAGG CTCAAGCCAT CTCTGTCCTC
241      CATGAGATGA TCCAGCAGAC CTTCAACCTC TTCAGCACAA AGGACTCATC TGCTGCTTTG
301      GATGAGACCC TTCTAGATGA ATTCTACATC GAACTTGACC AGCAGCTGAA TGACCTGGAG
361      TCCTGTGTGA TGCAGGAAGT GGGGGTGATA GAGTCTCCCC TGATGTACGA GGACTCCATC
421      CTGGCTGTGA GGAAATACTT CCAAAGAATC ACTCTATATC TGACAGAGAA GAAATACAGC
481      TCTTGTGCCT GGGAGGTTGT CAGAGCAGAA ATCATGAGAT CCTTCTCTTT ATCAATCAAC
541      TTGCAAAAAA GATTGAAGAG TAAGGAATGA

Quality Control:   cDNA sequencing results is available upon request.