IFNAR2 / IFNABR 단백질(Protein) (His Tag)

Interferon alpha/beta Receptor 2 ( IFNAR2 / IFNABR ) Protein

IFNAR2 / IFNABR Protein Product Information

• Synonym: IFN-R, IFN-alpha-REC, IFNABR, IFNARB
• Protein Construction: A DNA sequence encoding the extracellular domain of human IFNAR2 isoform a ( NP_997468.1 ) ( Met 1 - Lys 243 ) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

IFNAR2 / IFNABR Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ile 27
• Molecular Mass: The recombinant human IFNAR2 consists of 228 amino acids and predicts a molecular mass of 26.2 kDa. As a result of glycosylation, the rh IFNAR2 migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: IFNAR2 protein

IFNAR2 / IFNABR Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFNAR2 / IFNABR Protein Description

Interferon alpha/beta receptor 2, also known as IFN-alpha/beta receptor 2, Type I interferon receptor 2, IFNAR2 and IFNABR, is a single-pass type I membrane protein which belongs to the type II cytokine receptor family. IFNAR2 is a receptor for interferons alpha and beta. IFNAR2 was detected in most lymphocytes, monocytes, and granulocytes, although IFNAR2 expression was higher in the monocytes and granulocytes than in the lymphocytes. The intra- and interdaily variations of IFNAR2 in lymphocytes, monocytes, and granulocytes were small. Among the lymphocyte subsets, IFNAR2 showed high expression in natural killer (NK) cells and low expression in T lymphocytes. Isoform 1 and isoform 3 of IFNAR2 are directly involved in signal transduction due to their interaction with the TYR kinase, JAK1. Isoform 1 also interacts with the transcriptional factors, STAT1 and STAT2. Both forms are potent inhibitors of type I IFN activity. Defects in IFNAR2 are associated with susceptibility to hepatitis B virus infection (HBV infection). Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.

References

1. Novick D. et al.,1995, J. Leukoc. Biol. 57:712-18.
2. Chill JH. et al., 2003, Structure 11:791-802.
3. Tochizawa,S. et al., 2004, J Pharmacol Toxicol Methods. 50 (1):59-66.
4. Frodsham A.J. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 9148-53.