휴먼(Human) IGFBP3 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10430-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_000598.4

cDNA Size:   876

Synonym:  IBP3; BP-53; IGFBP3

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens insulin-like growth factor binding protein 3 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCAGCGGG CGCGACCCAC GCTCTGGGCC GCTGCGCTGA CTCTGCTGGT GCTGCTCCGC
61       GGGCCGCCGG TGGCGCGGGC TGGCGCGAGC TCGGCGGGCT TGGGTCCCGT GGTGCGCTGC
121      GAGCCGTGCG ACGCGCGTGC ACTGGCCCAG TGCGCGCCTC CGCCCGCCGT GTGCGCGGAG
181      CTGGTGCGCG AGCCGGGCTG CGGCTGCTGC CTGACGTGCG CACTGAGCGA GGGCCAGCCG
241      TGCGGCATCT ACACCGAGCG CTGTGGCTCC GGCCTTCGCT GCCAGCCGTC GCCCGACGAG
301      GCGCGACCGC TGCAGGCGCT GCTGGACGGC CGCGGGCTCT GCGTCAACGC TAGTGCCGTC
361      AGCCGCCTGC GCGCCTACCT GCTGCCAGCG CCGCCAGCTC CAGGAAATGC TAGTGAGTCG
421      GAGGAAGACC GCAGCGCCGG CAGTGTGGAG AGCCCGTCCG TCTCCAGCAC GCACCGGGTG
481      TCTGATCCCA AGTTCCACCC CCTCCATTCA AAGATAATCA TCATCAAGAA AGGGCATGCT
541      AAAGACAGCC AGCGCTACAA AGTTGACTAC GAGTCTCAGA GCACAGATAC CCAGAACTTC
601      TCCTCCGAGT CCAAGCGGGA GACAGAATAT GGTCCCTGCC GTAGAGAAAT GGAAGACACA
661      CTGAATCACC TGAAGTTCCT CAATGTGCTG AGTCCCAGGG GTGTACACAT TCCCAACTGT
721      GACAAGAAGG GATTTTATAA GAAAAAGCAG TGTCGCCCTT CCAAAGGCAG GAAGCGGGGC
781      TTCTGCTGGT GTGTGGATAA GTATGGGCAG CCTCTCCCAG GCTACACCAC CAAGGGGAAG
841      GAGGACGTGC ACTGCTACAG CATGCAGAGC AAGTAG

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) IGFBP3 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)