마우스(Mouse) IL1F1/IL1α 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

IL1A cDNA Clone Datasheet

IL1A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010554.4
• cDNA Size: 813
• Gene Synonym: Il-1a
• Source: Mouse

IL1A cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus interleukin 1 alpha DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 537C/T not causing the amino acid variation.

IL1A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL1A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

IL1A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAAAG TTCCTGACTT GTTTGAAGAC CTAAAGAACT GTTACAGTGA AAACGAAGAC
61       TACAGTTCTG CCATTGACCA TCTCTCTCTG AATCAGAAAT CCTTCTATGA TGCAAGCTAT
121      GGCTCACTTC ATGAGACTTG CACAGATCAG TTTGTATCTC TGAGAACCTC TGAAACGTCA
181      AAGATGTCCA ACTTCACCTT CAAGGAGAGC CGGGTGACAG TATCAGCAAC GTCAAGCAAC
241      GGGAAGATTC TGAAGAAGAG ACGGCTGAGT TTCAGTGAGA CCTTCACTGA AGATGACCTG
301      CAGTCCATAA CCCATGATCT GGAAGAGACC ATCCAACCCA GATCAGCACC TTACACCTAC
361      CAGAGTGATT TGAGATACAA ACTGATGAAG CTCGTCAGGC AGAAGTTTGT CATGAATGAT
421      TCCCTCAACC AAACTATATA TCAGGATGTG GACAAACACT ATCTCAGCAC CACTTGGTTA
481      AATGACCTGC AACAGGAAGT AAAATTTGAC ATGTATGCCT ACTCGTCGGG AGGAGATGAC
541      TCTAAATATC CTGTTACTCT AAAAATCTCA GATTCACAAC TGTTCGTGAG CGCTCAAGGA
601      GAAGACCAGC CCGTGTTGCT GAAGGAGTTG CCAGAAACAC CAAAACTCAT CACAGGTAGT
661      GAGACCGACC TCATTTTCTT CTGGAAAAGT ATCAACTCTA AGAACTACTT CACATCAGCT
721      GCTTATCCAG AGCTGTTTAT TGCCACCAAA GAACAAAGTC GGGTGCACCT GGCACGGGGA
781      CTGCCCTCTA TGACAGACTT CCAGATATCA TAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).