휴먼(Human) IL10Ra 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10419-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_001558.2

cDNA Size:   1737

Synonym:  IL10R, CDW210A, HIL-10R, IL-10R1, IL10RA

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens interleukin 10 receptor, alpha (IL10RA) DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutations: 180A/G and 459A/G, neither of which results in the amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCTGCCGT GCCTCGTAGT GCTGCTGGCG GCGCTCCTCA GCCTCCGTCT TGGCTCAGAC
61       GCTCATGGGA CAGAGCTGCC CAGCCCTCCG TCTGTGTGGT TTGAAGCAGA ATTTTTCCAC
121      CACATCCTCC ACTGGACACC CATCCCAAAT CAGTCTGAAA GTACCTGCTA TGAAGTGGCG
181      CTCCTGAGGT ATGGAATAGA GTCCTGGAAC TCCATCTCCA ACTGTAGCCA GACCCTGTCC
241      TATGACCTTA CCGCAGTGAC CTTGGACCTG TACCACAGCA ATGGCTACCG GGCCAGAGTG
301      CGGGCTGTGG ACGGCAGCCG GCACTCCAAC TGGACCGTCA CCAACACCCG CTTCTCTGTG
361      GATGAAGTGA CTCTGACAGT TGGCAGTGTG AACCTAGAGA TCCACAATGG CTTCATCCTC
421      GGGAAGATTC AGCTACCCAG GCCCAAGATG GCCCCCGCGA ATGACACATA TGAAAGCATC
481      TTCAGTCACT TCCGAGAGTA TGAGATTGCC ATTCGCAAGG TGCCGGGAAA CTTCACGTTC
541      ACACACAAGA AAGTAAAACA TGAAAACTTC AGCCTCCTAA CCTCTGGAGA AGTGGGAGAG
601      TTCTGTGTCC AGGTGAAACC ATCTGTCGCT TCCCGAAGTA ACAAGGGGAT GTGGTCTAAA
661      GAGGAGTGCA TCTCCCTCAC CAGGCAGTAT TTCACCGTGA CCAACGTCAT CATCTTCTTT
721      GCCTTTGTCC TGCTGCTCTC CGGAGCCCTC GCCTACTGCC TGGCCCTCCA GCTGTATGTG
781      CGGCGCCGAA AGAAGCTACC CAGTGTCCTG CTCTTCAAGA AGCCCAGCCC CTTCATCTTC
841      ATCAGCCAGC GTCCCTCCCC AGAGACCCAA GACACCATCC ACCCGCTTGA TGAGGAGGCC
901      TTTTTGAAGG TGTCCCCAGA GCTGAAGAAC TTGGACCTGC ACGGCAGCAC AGACAGTGGC
961      TTTGGCAGCA CCAAGCCATC CCTGCAGACT GAAGAGCCCC AGTTCCTCCT CCCTGACCCT
1021     CACCCCCAGG CTGACAGAAC GCTGGGAAAC GGGGAGCCCC CTGTGCTGGG GGACAGCTGC
1081     AGTAGTGGCA GCAGCAATAG CACAGACAGC GGGATCTGCC TGCAGGAGCC CAGCCTGAGC
1141     CCCAGCACAG GGCCCACCTG GGAGCAACAG GTGGGGAGCA ACAGCAGGGG CCAGGATGAC
1201     AGTGGCATTG ACTTAGTTCA AAACTCTGAG GGCCGGGCTG GGGACACACA GGGTGGCTCG
1261     GCCTTGGGCC ACCACAGTCC CCCGGAGCCT GAGGTGCCTG GGGAAGAAGA CCCAGCTGCT
1321     GTGGCATTCC AGGGTTACCT GAGGCAGACC AGATGTGCTG AAGAGAAGGC AACCAAGACA
1381     GGCTGCCTGG AGGAAGAATC GCCCTTGACA GATGGCCTTG GCCCCAAATT CGGGAGATGC
1441     CTGGTTGATG AGGCAGGCTT GCATCCACCA GCCCTGGCCA AGGGCTATTT GAAACAGGAT
1501     CCTCTAGAAA TGACTCTGGC TTCCTCAGGG GCCCCAACGG GACAGTGGAA CCAGCCCACT
1561     GAGGAATGGT CACTCCTGGC CTTGAGCAGC TGCAGTGACC TGGGAATATC TGACTGGAGC
1621     TTTGCCCATG ACCTTGCCCC TCTAGGCTGT GTGGCAGCCC CAGGTGGTCT CCTGGGCAGC
1681     TTTAACTCAG ACCTGGTCAC CCTGCCCCTC ATCTCTAGCC TGCAGTCAAG TGAGTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) IL10Ra 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)